1r4v
From Proteopedia
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| - | [[Image:1r4v.jpg|left|200px]] | + | [[Image:1r4v.jpg|left|200px]] |
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| - | '''1.9A crystal structure of protein AQ328 from Aquifex aeolicus''' | + | {{Structure |
| + | |PDB= 1r4v |SIZE=350|CAPTION= <scene name='initialview01'>1r4v</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CAC:CACODYLATE ION'>CAC</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= AQ_328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | }} | ||
| + | |||
| + | '''1.9A crystal structure of protein AQ328 from Aquifex aeolicus''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R4V is a [ | + | 1R4V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4V OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold., Qiu Y, Tereshko V, Kim Y, Zhang R, Collart F, Yousef M, Kossiakoff A, Joachimiak A, Proteins. 2006 Jan 1;62(1):8-16. PMID:[http:// | + | The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold., Qiu Y, Tereshko V, Kim Y, Zhang R, Collart F, Yousef M, Kossiakoff A, Joachimiak A, Proteins. 2006 Jan 1;62(1):8-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16287087 16287087] |
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: histon fold]] | [[Category: histon fold]] | ||
[[Category: mcsg]] | [[Category: mcsg]] | ||
| - | [[Category: midwest center for structural | + | [[Category: midwest center for structural genomic]] |
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:46:52 2008'' |
Revision as of 11:46, 20 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | AQ_328 (Aquifex aeolicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
1.9A crystal structure of protein AQ328 from Aquifex aeolicus
Overview
The structure of Aq_328, an uncharacterized protein from hyperthermophilic bacteria Aquifex aeolicus, has been determined to 1.9 A by using multi-wavelength anomalous diffraction (MAD) phasing. Although the amino acid sequence analysis shows that Aq_328 has no significant similarity to proteins with a known structure and function, the structure comparison by using the Dali server reveals that it: (1) assumes a histone-like fold, and (2) is similar to an ancestral nuclear histone protein (PDB code 1F1E) with z-score 8.1 and RMSD 3.6 A over 124 residues. A sedimentation equilibrium experiment indicates that Aq_328 is a monomer in solution, with an average sedimentation coefficient of 2.4 and an apparent molecular weight of about 20 kDa. The overall architecture of Aq_328 consists of two noncanonical histone domains in tandem repeat within a single chain, and is similar to eukaryotic heterodimer (H2A/H2B and H3/H4) and an archaeal histone heterodimer (HMfA/HMfB). The sequence comparisons between the two histone domains of Aq_328 and six eukaryotic/archaeal histones demonstrate that most of the conserved residues that underlie the Aq_328 architecture are used to build and stabilize the two cross-shaped antiparallel histone domains. The high percentage of salt bridges in the structure could be a factor in the protein's thermostability. The structural similarities to other histone-like proteins, molecular properties, and potential function of Aq_328 are discussed in this paper.
About this Structure
1R4V is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold., Qiu Y, Tereshko V, Kim Y, Zhang R, Collart F, Yousef M, Kossiakoff A, Joachimiak A, Proteins. 2006 Jan 1;62(1):8-16. PMID:16287087
Page seeded by OCA on Thu Mar 20 13:46:52 2008
Categories: Aquifex aeolicus | Single protein | Collart, F. | Joachimiak, A. | Kim, Y. | Kossiakoff, A. | MCSG, Midwest Center for Structural Genomics. | Qiu, Y. | Tereshko, V. | Zhang, R. | CAC | ZN | All-alpha | Histon fold | Mcsg | Midwest center for structural genomic | Protein structure initiative | Psi | Structural genomic
