3qk4
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of d(CGCGGGTACCCGCG)2 as A-DNA duplex== | |
| - | + | <StructureSection load='3qk4' size='340' side='right' caption='[[3qk4]], [[Resolution|resolution]] 2.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3qk4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QK4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qk4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qk4 RCSB], [http://www.ebi.ac.uk/pdbsum/3qk4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We report the 2.6A resolution crystal structure of the tetra-decamer d(CGCGGGTACCCGCG) in the tetragonal space group P4(3). This sequence contains the KpnI restriction site GGTACC in the centre which is flanked by alternating 'CG' sequences, and has a 'TA' step at the centre. These are features could favour the left-handed Z type helix. Despite this, overall the molecule has the A form. This is the first tetra-decamer crystallized in the A-DNA conformation, i.e. more than one full turn of the A helix. The crystallographic asymmetric unit consists of one tetra-decamer duplex. The helical twist and slide, as well as the base pair-base pair stacking interactions show alternations at the alternating pyrimidine-purine and purine-pyrimidine base steps. This variation is reminiscent of the dinucleotide repeat in left-handed Z-DNA helices. The crystal packing is unlike other A-DNA crystal structures, with each helix having a large number of contacts of many different types with symmetry-related neighbours. | ||
| - | + | The structure of a full turn of an A-DNA duplex d(CGCGGGTACCCGCG)(2).,Venkadesh S, Mandal PK, Gautham N Biochem Biophys Res Commun. 2011 Apr 8;407(2):307-12. Epub 2011 Mar 21. PMID:21397589<ref>PMID:21397589</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Gautham, N | + | == References == |
| - | [[Category: Mandal, P K | + | <references/> |
| - | [[Category: Venkadesh, S | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Gautham, N]] | ||
| + | [[Category: Mandal, P K]] | ||
| + | [[Category: Venkadesh, S]] | ||
[[Category: A-dna duplex]] | [[Category: A-dna duplex]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
Revision as of 11:04, 19 December 2014
Crystal structure of d(CGCGGGTACCCGCG)2 as A-DNA duplex
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