1r6v
From Proteopedia
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| - | [[Image:1r6v.gif|left|200px]] | + | [[Image:1r6v.gif|left|200px]] |
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| - | '''Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin''' | + | {{Structure |
| + | |PDB= 1r6v |SIZE=350|CAPTION= <scene name='initialview01'>1r6v</scene>, resolution 1.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R6V is a [ | + | 1R6V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Fervidobacterium_pennivorans Fervidobacterium pennivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R6V OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin., Kim JS, Kluskens LD, de Vos WM, Huber R, van der Oost J, J Mol Biol. 2004 Jan 16;335(3):787-97. PMID:[http:// | + | Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin., Kim JS, Kluskens LD, de Vos WM, Huber R, van der Oost J, J Mol Biol. 2004 Jan 16;335(3):787-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14687574 14687574] |
[[Category: Fervidobacterium pennivorans]] | [[Category: Fervidobacterium pennivorans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: subtilisin]] | [[Category: subtilisin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:38 2008'' |
Revision as of 11:47, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin
Overview
Structure-forming fibrous proteins like keratins, gelatins and collagens are degraded only by a few proteases as their tight packing limits access to the potential cleavage sites. To understand the keratin degradation in detail, we describe the first crystal structure of a keratin-degrading enzyme (keratinase), fervidolysin, from Fervidobacterium pennivorans as an immature form with propeptide (PD)-bound. The 1.7A resolution crystal structure shows that the protease is composed of four domains: a catalytic domain (CD), two beta-sandwich domains (SDs), and the PD domain. A structural alignment shows a distant relationship between the PD-CD substructure of fervidolysin and pro-subtilisin E. Tight binding of PD to the remaining part of the protease is mediated by hydrogen bonds along the domain surfaces and around the active cleft, and by the clamps to SD1 and SD2. The crystal structure of this multi-domain protein fervidolysin provides insights into proenzyme activation and the role of non-catalytic domains, suggesting a functional relationship to the fibronectin (FN)-like domains of the human promatrix metalloprotease-2 (proMMP-2) that degrades the fibrous polymeric substrate gelatin.
About this Structure
1R6V is a Single protein structure of sequence from Fervidobacterium pennivorans. Full crystallographic information is available from OCA.
Reference
Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin., Kim JS, Kluskens LD, de Vos WM, Huber R, van der Oost J, J Mol Biol. 2004 Jan 16;335(3):787-97. PMID:14687574
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