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3w6f

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{{STRUCTURE_3w6f| PDB=3w6f | SCENE= }}
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==Crystal structure of catalytic domain of chitinase from Ralstonia sp. A-471 (E162Q) in complex with disaccharide==
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===Crystal structure of catalytic domain of chitinase from Ralstonia sp. A-471 (E162Q) in complex with disaccharide===
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<StructureSection load='3w6f' size='340' side='right' caption='[[3w6f]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23658014}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3w6f]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ralstonia_sp._a-471 Ralstonia sp. a-471]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W6F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W6F FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w6b|3w6b]], [[3w6c|3w6c]], [[3w6d|3w6d]], [[3w6e|3w6e]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w6f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w6f RCSB], [http://www.ebi.ac.uk/pdbsum/3w6f PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Chitinase C from Ralstonia sp. A-471 (Ra-ChiC) has a catalytic domain sequence similar to goose-type (G-type) lysozymes and, unlike other chitinases, belongs to glycohydrolase (GH) family 23. Using NMR spectroscopy, however, Ra-ChiC was found to interact only with the chitin dimer but not with the peptidoglycan fragment. Here we report the crystal structures of wild-type, E141Q, and E162Q of the catalytic domain of Ra-ChiC with or without chitin oligosaccharides. Ra-ChiC has a substrate-binding site including a tunnel-shaped cavity, which determines the substrate specificity. Mutation analyses based on this structural information indicated that a highly conserved Glu-141 acts as a catalytic acid, and that Asp-226 located at the roof of the tunnel activates a water molecule as a catalytic base. The unique arrangement of the catalytic residues makes a clear contrast to the other GH23 members and also to inverting GH19 chitinases.
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==About this Structure==
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Crystal Structures of the Catalytic Domain of a Novel Glycohydrolase Family 23 Chitinase from Ralstonia sp. A-471 Reveals a Unique Arrangement of the Catalytic Residues for Inverting Chitin Hydrolysis.,Arimori T, Kawamoto N, Shinya S, Okazaki N, Nakazawa M, Miyatake K, Fukamizo T, Ueda M, Tamada T J Biol Chem. 2013 Jun 28;288(26):18696-706. doi: 10.1074/jbc.M113.462135. Epub, 2013 May 8. PMID:23658014<ref>PMID:23658014</ref>
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[[3w6f]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ralstonia_sp._a-471 Ralstonia sp. a-471]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W6F OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023658014</ref><references group="xtra"/><references/>
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</div>
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==See Also==
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*[[Chitinase|Chitinase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Chitinase]]
[[Category: Chitinase]]
[[Category: Ralstonia sp. a-471]]
[[Category: Ralstonia sp. a-471]]
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[[Category: Arimori, T.]]
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[[Category: Arimori, T]]
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[[Category: Fukamizo, T.]]
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[[Category: Fukamizo, T]]
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[[Category: Kawamoto, N.]]
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[[Category: Kawamoto, N]]
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[[Category: Miyatake, K.]]
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[[Category: Miyatake, K]]
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[[Category: Nakazawa, M.]]
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[[Category: Nakazawa, M]]
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[[Category: Okazaki, N.]]
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[[Category: Okazaki, N]]
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[[Category: Tamada, T.]]
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[[Category: Tamada, T]]
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[[Category: Ueda, M.]]
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[[Category: Ueda, M]]
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[[Category: Chitinase]]
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[[Category: Enzyme]]
[[Category: Enzyme]]
[[Category: Gh family 23]]
[[Category: Gh family 23]]
[[Category: Glycoside hydrolase]]
[[Category: Glycoside hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 06:34, 21 December 2014

Crystal structure of catalytic domain of chitinase from Ralstonia sp. A-471 (E162Q) in complex with disaccharide

3w6f, resolution 2.10Å

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