1r73
From Proteopedia
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| - | [[Image:1r73.gif|left|200px]] | + | [[Image:1r73.gif|left|200px]] |
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| - | '''Solution Structure of TM1492, the L29 ribosomal protein from Thermotoga maritima''' | + | {{Structure |
| + | |PDB= 1r73 |SIZE=350|CAPTION= <scene name='initialview01'>1r73</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution Structure of TM1492, the L29 ribosomal protein from Thermotoga maritima''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R73 is a [ | + | 1R73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R73 OCA]. |
==Reference== | ==Reference== | ||
| - | NMR for structural proteomics of Thermotoga maritima: screening and structure determination., Peti W, Etezady-Esfarjani T, Herrmann T, Klock HE, Lesley SA, Wuthrich K, J Struct Funct Genomics. 2004;5(3):205-15. PMID:[http:// | + | NMR for structural proteomics of Thermotoga maritima: screening and structure determination., Peti W, Etezady-Esfarjani T, Herrmann T, Klock HE, Lesley SA, Wuthrich K, J Struct Funct Genomics. 2004;5(3):205-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15263836 15263836] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
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[[Category: Wuethrich, K.]] | [[Category: Wuethrich, K.]] | ||
[[Category: jcsg]] | [[Category: jcsg]] | ||
| - | [[Category: joint center for structural | + | [[Category: joint center for structural genomic]] |
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: ribosome]] | [[Category: ribosome]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:45 2008'' |
Revision as of 11:47, 20 March 2008
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Solution Structure of TM1492, the L29 ribosomal protein from Thermotoga maritima
Overview
This paper describes the NMR screening of 141 small (<15 kDa) recombinant Thermotoga maritima proteins for globular folding. The experimental data shows that approximately 25% of the screened proteins are folded under our screening conditions, which makes this procedure an important step for selecting those proteins that are suitable for structure determination. A comparison of screening based either on 1D 1H NMR with unlabeled proteins or on 2D [1H,15N]-COSY with uniformly 15N-labeled proteins is presented, and a comprehensive analysis of the 1D 1H NMR screening data is described. As an illustration of the utility of these methods to structural proteomics, the NMR structure determination of TM1492 (ribosomal protein L29) is presented. This 66-residue protein consists of a N-terminal 3(10)-helix and two long alpha-helices connected by a tight turn centered about glycine 35, where conserved leucine and isoleucine residues in the two alpha-helices form a small hydrophobic core.
About this Structure
1R73 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
Reference
NMR for structural proteomics of Thermotoga maritima: screening and structure determination., Peti W, Etezady-Esfarjani T, Herrmann T, Klock HE, Lesley SA, Wuthrich K, J Struct Funct Genomics. 2004;5(3):205-15. PMID:15263836
Page seeded by OCA on Thu Mar 20 13:47:45 2008
Categories: Single protein | Thermotoga maritima | Etezady-Esfarjani, T. | Herrmann, T. | JCSG, Joint Center for Structural Genomics. | Klock, H E. | Lesley, S A. | Peti, W. | Wuethrich, K. | Jcsg | Joint center for structural genomic | Protein structure initiative | Psi | Ribosome | Structural genomic
