3w0l
From Proteopedia
(Difference between revisions)
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| - | + | ==The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex== | |
| - | + | <StructureSection load='3w0l' size='340' side='right' caption='[[3w0l]], [[Resolution|resolution]] 2.92Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3w0l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W0L FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gck ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), gckr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w0l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w0l RCSB], [http://www.ebi.ac.uk/pdbsum/3w0l PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glucokinase (GK) is a monomeric allosteric enzyme and plays a pivotal role in blood glucose homeostasis. GK is regulated by GK regulatory protein (GKRP), and indirectly by allosteric effectors of GKRP. Despite the critical roles of GK and GKRP, the molecular basis for the allosteric regulation mechanism of GK by GKRP remains unclear. We determined the crystal structure of Xenopus GK and GKRP complex in the presence of fructose-6-phosphate at 2.9 A. GKRP binds to a super-open conformation of GK mainly through hydrophobic interaction, inhibiting the GK activity by locking a small domain of GK. We demonstrate the molecular mechanism for the modulation of GK activity by allosteric effectors of GKRP. Importantly, GKRP releases GK in a sigmoidal manner in response to glucose concentration by restricting a structural rearrangement of the GK small domain via a single ion pair. We find that GKRP acts as an allosteric switch for GK in blood glucose control by the liver. | ||
| - | + | Molecular basis for the role of glucokinase regulatory protein as the allosteric switch for glucokinase.,Choi JM, Seo MH, Kyeong HH, Kim E, Kim HS Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10171-6. doi:, 10.1073/pnas.1300457110. Epub 2013 Jun 3. PMID:23733961<ref>PMID:23733961</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Glucokinase Regulatory Protein|Glucokinase Regulatory Protein]] | |
| + | *[[Hexokinase|Hexokinase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Glucokinase]] | [[Category: Glucokinase]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
| - | [[Category: Choi, J M | + | [[Category: Choi, J M]] |
| - | [[Category: Kim, E | + | [[Category: Kim, E]] |
| - | [[Category: Kim, H S | + | [[Category: Kim, H S]] |
| - | [[Category: Kyeong, H H | + | [[Category: Kyeong, H H]] |
| - | [[Category: Seo, M H | + | [[Category: Seo, M H]] |
[[Category: Aba sandwich]] | [[Category: Aba sandwich]] | ||
[[Category: Kinase]] | [[Category: Kinase]] | ||
Revision as of 06:58, 21 December 2014
The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex
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