3vk6

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{{STRUCTURE_3vk6| PDB=3vk6 | SCENE= }}
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==Crystal structure of a phosphotyrosine binding domain==
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===Crystal structure of a phosphotyrosine binding domain===
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<StructureSection load='3vk6' size='340' side='right' caption='[[3vk6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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{{ABSTRACT_PUBMED_22252131}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3vk6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VK6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cbll1, Hakai ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vk6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3vk6 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phosphotyrosine-binding domains, typified by the SH2 (Src homology 2) and PTB domains, are critical upstream components of signal transduction pathways. The E3 ubiquitin ligase Hakai targets tyrosine-phosphorylated E-cadherin via an uncharacterized domain. In this study, the crystal structure of Hakai (amino acids 106-206) revealed that it forms an atypical, zinc-coordinated homodimer by utilizing residues from the phosphotyrosine-binding domain of two Hakai monomers. Hakai dimerization allows the formation of a phosphotyrosine-binding pocket that recognizes specific phosphorylated tyrosines and flanking acidic amino acids of Src substrates, such as E-cadherin, cortactin and DOK1. NMR and mutational analysis identified the Hakai residues required for target binding within the binding pocket, now named the HYB domain. ZNF645 also possesses a HYB domain but demonstrates different target specificities. The HYB domain is structurally different from other phosphotyrosine-binding domains and is a potential drug target due to its novel structural features.
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==Function==
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Structure of a novel phosphotyrosine-binding domain in Hakai that targets E-cadherin.,Mukherjee M, Chow SY, Yusoff P, Seetharaman J, Ng C, Sinniah S, Koh XW, Asgar NF, Li D, Yim D, Jackson RA, Yew J, Qian J, Iyu A, Lim YP, Zhou X, Sze SK, Guy GR, Sivaraman J EMBO J. 2012 Jan 17;31(5):1308-19. doi: 10.1038/emboj.2011.496. PMID:22252131<ref>PMID:22252131</ref>
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[[http://www.uniprot.org/uniprot/HAKAI_MOUSE HAKAI_MOUSE]] Promotes ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1. Targets CDH1 for endocytosis and degradation.<ref>PMID:11836526</ref> <ref>PMID:22252131</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3vk6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VK6 OCA].
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</div>
==See Also==
==See Also==
*[[Ubiquitin protein ligase|Ubiquitin protein ligase]]
*[[Ubiquitin protein ligase|Ubiquitin protein ligase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022252131</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Mukherjee, M.]]
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[[Category: Mukherjee, M]]
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[[Category: Sivaraman, J.]]
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[[Category: Sivaraman, J]]
[[Category: Hyb]]
[[Category: Hyb]]
[[Category: Ligase]]
[[Category: Ligase]]
[[Category: Phosphotyrosine binding domain]]
[[Category: Phosphotyrosine binding domain]]

Revision as of 07:16, 21 December 2014

Crystal structure of a phosphotyrosine binding domain

3vk6, resolution 1.90Å

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