3uf9

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{{STRUCTURE_3uf9| PDB=3uf9 | SCENE= }}
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==Crystal structure of SsoPox in complex with the phosphotriester fensulfothion==
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===Crystal structure of SsoPox in complex with the phosphotriester fensulfothion===
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<StructureSection load='3uf9' size='340' side='right' caption='[[3uf9]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23139857}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3uf9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UF9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UF9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FST:O,O-DIETHYL+O-{4-[(R)-METHYLSULFINYL]PHENYL}+PHOSPHOROTHIOATE'>FST</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vc5|2vc5]], [[2vc7|2vc7]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">php, SSO2522 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uf9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uf9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uf9 RCSB], [http://www.ebi.ac.uk/pdbsum/3uf9 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SsoPox is a lactonase endowed with promiscuous phosphotriesterase activity isolated from Sulfolobus solfataricus that belongs to the Phosphotriesterase-Like Lactonase family. Because of its intrinsic thermal stability, SsoPox is seen as an appealing candidate as a bioscavenger for organophosphorus compounds. A comprehensive kinetic characterisation of SsoPox has been performed with various phosphotriesters (insecticides) and phosphodiesters (nerve agent analogues) as substrates. We show that SsoPox is active for a broad range of OPs and remains active under denaturing conditions. In addition, its OP hydrolase activity is highly stimulated by anionic detergent at ambient temperature and exhibits catalytic efficiencies as high as k(cat)/K(M) of 10(5) M(-1)s(-1) against a nerve agent analogue. The structure of SsoPox bound to the phosphotriester fensulfothion reveals an unexpected and non-productive binding mode. This feature suggests that SsoPox's active site is sub-optimal for phosphotriester binding, which depends not only upon shape but also on localised charge of the ligand.
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==Function==
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Characterisation of the organophosphate hydrolase catalytic activity of SsoPox.,Hiblot J, Gotthard G, Chabriere E, Elias M Sci Rep. 2012;2:779. doi: 10.1038/srep00779. Epub 2012 Nov 8. PMID:23139857<ref>PMID:23139857</ref>
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[[http://www.uniprot.org/uniprot/PHP_SULSO PHP_SULSO]] Has a low paraoxonase activity. Also active, but with a lower activity, against other oregano-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.<ref>PMID:15909078</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3uf9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UF9 OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023139857</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Aryldialkylphosphatase]]
[[Category: Aryldialkylphosphatase]]
[[Category: Sulfolobus solfataricus]]
[[Category: Sulfolobus solfataricus]]
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[[Category: Chabriere, E.]]
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[[Category: Chabriere, E]]
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[[Category: Elias, M.]]
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[[Category: Elias, M]]
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[[Category: Gotthard, G.]]
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[[Category: Gotthard, G]]
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[[Category: Hiblot, J.]]
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[[Category: Hiblot, J]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Lactonase]]
[[Category: Lactonase]]

Revision as of 07:20, 21 December 2014

Crystal structure of SsoPox in complex with the phosphotriester fensulfothion

3uf9, resolution 2.68Å

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