3vm5
From Proteopedia
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| - | + | ==Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris== | |
| - | === | + | <StructureSection load='3vm5' size='340' side='right' caption='[[3vm5]], [[Resolution|resolution]] 2.85Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3vm5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VM5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VM5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vm5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vm5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vm5 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The medaka fish alpha-amylase was expressed and purified. The expression systems were constructed using methylotrophic yeast Pichia pastoris, and the recombinant proteins were secreted into the culture medium. Purified recombinant alpha-amylase exhibited starch hydrolysis activity. The optimal pH, denaturation temperature, and K(M) and V(max) values were determined; chloride ions were essential for enzyme activity. The purified protein was also crystallized and examined by X-ray crystallography. The structure has the (alpha/beta)(8) barrel fold, as do other known alpha-amylases, and the overall structure is very similar to the structure of vertebrate (human and pig) alpha-amylases. A novel expression plasmid was developed. Using this plasmid, high-throughput construction of an expression system by homologous recombination in P. pastoris cells, previously reported for membrane proteins, was successfully applied to the secretory protein. | ||
| - | + | Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris.,Mizutani K, Toyoda M, Otake Y, Yoshioka S, Takahashi N, Mikami B Biochim Biophys Acta. 2012 May 18. PMID:22613096<ref>PMID:22613096</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Amylase|Amylase]] | ||
| + | *[[User:Gabriel Pons/Sandbox 2|User:Gabriel Pons/Sandbox 2]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Oryzias latipes]] | [[Category: Oryzias latipes]] | ||
| - | [[Category: Mikami, B | + | [[Category: Mikami, B]] |
| - | [[Category: Mizutani, K | + | [[Category: Mizutani, K]] |
| - | [[Category: Toyoda, M | + | [[Category: Toyoda, M]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Starch hydrolysis]] | [[Category: Starch hydrolysis]] | ||
Revision as of 07:36, 21 December 2014
Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris
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