1rev
From Proteopedia
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| - | [[Image:1rev.gif|left|200px]] | + | [[Image:1rev.gif|left|200px]] |
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| - | '''HIV-1 REVERSE TRANSCRIPTASE''' | + | {{Structure |
| + | |PDB= 1rev |SIZE=350|CAPTION= <scene name='initialview01'>1rev</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=TB9:4-CHLORO-8-METHYL-7-(3-METHYL-BUT-2-ENYL)-6,7,8,9-TETRAHYDRO-2H-2,7,9A-TRIAZA-BENZO[CD]AZULENE-1-THIONE'>TB9</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] | ||
| + | |GENE= HIV-1 POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1]) | ||
| + | }} | ||
| + | |||
| + | '''HIV-1 REVERSE TRANSCRIPTASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1REV is a [ | + | 1REV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1REV OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of HIV-1 reverse transcriptase complexed with 9-chloro-TIBO: lessons for inhibitor design., Ren J, Esnouf R, Hopkins A, Ross C, Jones Y, Stammers D, Stuart D, Structure. 1995 Sep 15;3(9):915-26. PMID:[http:// | + | The structure of HIV-1 reverse transcriptase complexed with 9-chloro-TIBO: lessons for inhibitor design., Ren J, Esnouf R, Hopkins A, Ross C, Jones Y, Stammers D, Stuart D, Structure. 1995 Sep 15;3(9):915-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8535785 8535785] |
[[Category: Human immunodeficiency virus 1]] | [[Category: Human immunodeficiency virus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: MG]] | [[Category: MG]] | ||
[[Category: TB9]] | [[Category: TB9]] | ||
| - | [[Category: | + | [[Category: aid]] |
[[Category: aspartyl protease]] | [[Category: aspartyl protease]] | ||
[[Category: endonuclease]] | [[Category: endonuclease]] | ||
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[[Category: polyprotein]] | [[Category: polyprotein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:50:37 2008'' |
Revision as of 11:50, 20 March 2008
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| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | HIV-1 POL (Human immunodeficiency virus 1) | ||||||
| Activity: | RNA-directed DNA polymerase, with EC number 2.7.7.49 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIV-1 REVERSE TRANSCRIPTASE
Overview
BACKGROUND: HIV reverse transcriptase (RT) is a key target of anti-AIDS therapies. Structural studies of HIV-1 RT, unliganded and complexed with different non-nucleoside inhibitors (NNIs), have pointed to a common mode of binding and inactivation through distortion of the polymerase catalytic site by NNIs containing two hinged rings. The mode of binding of the TIBO family of inhibitors is of interest because these compounds do not fit the two-hinged-ring model. RESULTS: The structure of HIV-1 RT complexed with 9-chloro-TIBO (R82913) has been determined at 2.6 A resolution. As reported for the lower resolution analysis of another TIBO compound, this inhibitor binds at the same site as other NNIs, but our higher resolution study reveals the Cl-TIBO is distorted from the conformation seen in crystals of the inhibitor alone. This allows Cl-TIBO to mimic the binding of NNIs containing two hinged rings. Inhibitor-protein interactions are again predominantly hydrophobic and the protein conformation corresponds to that seen in complexes with other tight-binding NNIs. CONCLUSIONS: Although Cl-TIBO is chemically very different from other NNIs, it achieves remarkable spatial equivalence and shape complementarity with other NNIs on binding to RT. Comparison of the different RT-NNI complexes suggests modifications to the TIBO group of inhibitors which might enhance their binding and hence, potentially, their therapeutic efficacy.
About this Structure
1REV is a Protein complex structure of sequences from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.
Reference
The structure of HIV-1 reverse transcriptase complexed with 9-chloro-TIBO: lessons for inhibitor design., Ren J, Esnouf R, Hopkins A, Ross C, Jones Y, Stammers D, Stuart D, Structure. 1995 Sep 15;3(9):915-26. PMID:8535785
Page seeded by OCA on Thu Mar 20 13:50:37 2008
Categories: Human immunodeficiency virus 1 | Protein complex | RNA-directed DNA polymerase | Esnouf, R. | Hopkins, A. | Jones, Y. | Ren, J. | Ross, C. | Stammers, D. | Stuart, D. | MG | TB9 | Aid | Aspartyl protease | Endonuclease | Hiv-1 reverse transcriptase | Hydrolase | Nucleotidyltransferase | Polyprotein
