4bry

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4bry| PDB=4bry | SCENE= }}
+
==The Idas:Geminin heterodimeric parallel coiled-coil==
-
===The Idas:Geminin heterodimeric parallel coiled-coil===
+
<StructureSection load='4bry' size='340' side='right' caption='[[4bry]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24064211}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4bry]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BRY FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bry OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bry RCSB], [http://www.ebi.ac.uk/pdbsum/4bry PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Geminin is an important regulator of proliferation and differentiation in metazoans, which predominantly inhibits the DNA replication licensing factor Cdt1, preventing genome over-replication. We show that Geminin preferentially forms stable coiled-coil heterodimers with its homologue, Idas. In contrast to Idas:Geminin heterodimers, Idas homodimers are thermodynamically unstable and are unlikely to exist as a stable macromolecule under physiological conditions. The crystal structure of the homology regions of Idas in complex with Geminin showed a tight head-to-head heterodimeric coiled-coil. This Idas:Geminin heterodimer binds Cdt1 less strongly than Geminin:Geminin, still with high affinity (~30nM), but with notably different thermodynamic properties. Consistently, in Xenopus egg extracts, Idas:Geminin is less active in licensing inhibition compared to a Geminin:Geminin homodimer. In human cultured cells, ectopic expression of Idas leads to limited over-replication, which is counteracted by Geminin co-expression. The properties of the Idas:Geminin complex suggest it as the functional form of Idas, and provide a possible mechanism to modulate Geminin activity.
-
==Function==
+
The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing.,Caillat C, Pefani ED, Gillespie PJ, Taraviras S, Blow JJ, Lygerou Z, Perrakis A J Biol Chem. 2013 Oct 2. PMID:24064211<ref>PMID:24064211</ref>
-
[[http://www.uniprot.org/uniprot/GEMI_HUMAN GEMI_HUMAN]] Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.<ref>PMID:9635433</ref> <ref>PMID:14993212</ref> <ref>PMID:22615398</ref> Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control.<ref>PMID:9635433</ref> <ref>PMID:14993212</ref> <ref>PMID:22615398</ref> [[http://www.uniprot.org/uniprot/MCIN_HUMAN MCIN_HUMAN]] Transcription regulator required for multiciliate cell differentiation. Acts by promoting transcription of genes required for multiciliate cell formation. Probably acts in a multiprotein complex (By similarity). Plays a role in mitotic cell cycle progression by promoting cell cycle exit.
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[4bry]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BRY OCA].
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:024064211</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: Human]]
[[Category: Human]]
-
[[Category: Caillat, C.]]
+
[[Category: Caillat, C]]
-
[[Category: Perrakis, A.]]
+
[[Category: Perrakis, A]]
[[Category: Cell cycle]]
[[Category: Cell cycle]]
[[Category: Dna replication licensing]]
[[Category: Dna replication licensing]]

Revision as of 08:53, 21 December 2014

The Idas:Geminin heterodimeric parallel coiled-coil

4bry, resolution 2.89Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bry"
Personal tools