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4btg

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{{STRUCTURE_4btg| PDB=4btg | SCENE= }}
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==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==
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===Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution===
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<StructureSection load='4btg' size='340' side='right' caption='[[4btg]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23891288}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The cystovirus varphi6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. varphi6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an alpha-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine.
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==Function==
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Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid.,Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC Structure. 2013 Aug 6;21(8):1374-83. doi: 10.1016/j.str.2013.06.007. Epub 2013, Jul 25. PMID:23891288<ref>PMID:23891288</ref>
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[[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023891288</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Pseudomonas phage phi6]]
[[Category: Pseudomonas phage phi6]]
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[[Category: Boura, E.]]
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[[Category: Boura, E]]
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[[Category: Cheng, N.]]
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[[Category: Cheng, N]]
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[[Category: Heymann, J B.]]
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[[Category: Heymann, J B]]
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[[Category: Hurley, J H.]]
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[[Category: Hurley, J H]]
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[[Category: Mindich, L.]]
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[[Category: Mindich, L]]
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[[Category: Nemecek, D.]]
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[[Category: Nemecek, D]]
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[[Category: Plevka, P.]]
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[[Category: Plevka, P]]
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[[Category: Qiao, J.]]
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[[Category: Qiao, J]]
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[[Category: Steven, A C.]]
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[[Category: Steven, A C]]
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[[Category: Wu, W.]]
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[[Category: Wu, W]]
[[Category: Cystoviridae]]
[[Category: Cystoviridae]]
[[Category: Flexible fitting]]
[[Category: Flexible fitting]]
[[Category: Procapsid structure]]
[[Category: Procapsid structure]]
[[Category: Virus]]
[[Category: Virus]]

Revision as of 09:00, 21 December 2014

Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution

4btg, resolution 4.40Å

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