1riq
From Proteopedia
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| - | [[Image:1riq.gif|left|200px]] | + | [[Image:1riq.gif|left|200px]] |
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| - | '''The crystal structure of the catalytic fragment of the alanyl-tRNA synthetase''' | + | {{Structure |
| + | |PDB= 1riq |SIZE=350|CAPTION= <scene name='initialview01'>1riq</scene>, resolution 2.14Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Alanine--tRNA_ligase Alanine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.7 6.1.1.7] | ||
| + | |GENE= ALAS, AQ_1293 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | }} | ||
| + | |||
| + | '''The crystal structure of the catalytic fragment of the alanyl-tRNA synthetase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RIQ is a [ | + | 1RIQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RIQ OCA]. |
==Reference== | ==Reference== | ||
| - | Alanyl-tRNA synthetase crystal structure and design for acceptor-stem recognition., Swairjo MA, Otero FJ, Yang XL, Lovato MA, Skene RJ, McRee DE, Ribas de Pouplana L, Schimmel P, Mol Cell. 2004 Mar 26;13(6):829-41. PMID:[http:// | + | Alanyl-tRNA synthetase crystal structure and design for acceptor-stem recognition., Swairjo MA, Otero FJ, Yang XL, Lovato MA, Skene RJ, McRee DE, Ribas de Pouplana L, Schimmel P, Mol Cell. 2004 Mar 26;13(6):829-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15053876 15053876] |
[[Category: Alanine--tRNA ligase]] | [[Category: Alanine--tRNA ligase]] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
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[[Category: Swairjo, M A.]] | [[Category: Swairjo, M A.]] | ||
[[Category: Yang, X L.]] | [[Category: Yang, X L.]] | ||
| - | [[Category: beta sheet and flanking | + | [[Category: beta sheet and flanking helice]] |
[[Category: class ii aminoacyl-trna synthetase]] | [[Category: class ii aminoacyl-trna synthetase]] | ||
[[Category: helix-loop-helix motif]] | [[Category: helix-loop-helix motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:52:10 2008'' |
Revision as of 11:52, 20 March 2008
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| , resolution 2.14Å | |||||||
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| Gene: | ALAS, AQ_1293 (Aquifex aeolicus) | ||||||
| Activity: | Alanine--tRNA ligase, with EC number 6.1.1.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of the catalytic fragment of the alanyl-tRNA synthetase
Overview
Early work on aminoacylation of alanine-specific tRNA (tRNA(Ala)) by alanyl-tRNA synthetase (AlaRS) gave rise to the concept of an early "second genetic code" imbedded in the acceptor stems of tRNAs. A single conserved and position-specific G:U base pair in the tRNA acceptor stem is the key identity determinant. Further understanding has been limited due to lack of a crystal structure of the enzyme. We determined a 2.14 A crystal structure of the 453 amino acid catalytic fragment of Aquifex aeolicus AlaRS. It contains the catalytic domain characteristic of class II synthetases, a helical domain with a hairpin motif critical for acceptor-stem recognition, and a C-terminal domain of a mixed alpha/beta fold. Docking of tRNA(Ala) on AlaRS shows critical contacts with the three domains, consistent with previous mutagenesis and functional data. It also suggests conformational flexibility within the C domain, which might allow for the positional variation of the key G:U base pair seen in some tRNA(Ala)s.
About this Structure
1RIQ is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Alanyl-tRNA synthetase crystal structure and design for acceptor-stem recognition., Swairjo MA, Otero FJ, Yang XL, Lovato MA, Skene RJ, McRee DE, Ribas de Pouplana L, Schimmel P, Mol Cell. 2004 Mar 26;13(6):829-41. PMID:15053876
Page seeded by OCA on Thu Mar 20 13:52:10 2008
