4a1r
From Proteopedia
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| - | + | ==The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.== | |
| - | + | <StructureSection load='4a1r' size='340' side='right' caption='[[4a1r]], [[Resolution|resolution]] 1.92Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4a1r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A1R FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a1r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a1r RCSB], [http://www.ebi.ac.uk/pdbsum/4a1r PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lip is a membrane-bound lipoprotein and a core component of the type VI secretion system found in Gram-negative bacteria. The structure of a Lip construct (residues 29-176) from Serratia marcescens (SmLip) has been determined at 1.92 A resolution. Experimental phases were derived using a single-wavelength anomalous dispersion approach on a sample cocrystallized with iodide. The membrane localization of the native protein was confirmed. The structure is that of the globular domain lacking only the lipoprotein signal peptide and the lipidated N-terminus of the mature protein. The protein fold is dominated by an eight-stranded beta-sandwich and identifies SmLip as a new member of the transthyretin family of proteins. Transthyretin and the only other member of the family fold, 5-hydroxyisourate hydrolase, form homotetramers important for their function. The asymmetric unit of SmLip is a tetramer with 222 symmetry, but the assembly is distinct from that previously noted for the transthyretin protein family. However, structural comparisons and bacterial two-hybrid data suggest that the SmLip tetramer is not relevant to its role as a core component of the type VI secretion system, but rather reflects a propensity for SmLip to participate in protein-protein interactions. A relatively low level of sequence conservation amongst Lip homologues is noted and is restricted to parts of the structure that might be involved in interactions with physiological partners. | ||
| - | + | The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system.,Rao VA, Shepherd SM, English G, Coulthurst SJ, Hunter WN Acta Crystallogr D Biol Crystallogr. 2011 Dec;67(Pt 12):1065-72. Epub 2011 Nov, 18. PMID:22120744<ref>PMID:22120744</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | < | + | </div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
| - | [[Category: Coulthurst, S J | + | [[Category: Coulthurst, S J]] |
| - | [[Category: English, G | + | [[Category: English, G]] |
| - | [[Category: Hunter, W N | + | [[Category: Hunter, W N]] |
| - | [[Category: Rao, V A | + | [[Category: Rao, V A]] |
| - | [[Category: Shepherd, S M | + | [[Category: Shepherd, S M]] |
[[Category: Beta-sandwich]] | [[Category: Beta-sandwich]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
[[Category: T6ss]] | [[Category: T6ss]] | ||
Revision as of 09:09, 21 December 2014
The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.
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