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4bt3

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{{STRUCTURE_4bt3| PDB=4bt3 | SCENE= }}
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==acetolactate decarboxylase with a bound (2R,3R)-2,3-Dihydroxy-2- methylbutanoic acid==
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===acetolactate decarboxylase with a bound (2R,3R)-2,3-Dihydroxy-2- methylbutanoic acid===
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<StructureSection load='4bt3' size='340' side='right' caption='[[4bt3]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23985082}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4bt3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BT3 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WTZ:(2R,3R)-2,3-DIHYDROXY-2-METHYLBUTANOIC+ACID'>WTZ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bt2|4bt2]], [[4bt4|4bt4]], [[4bt5|4bt5]], [[4bt6|4bt6]], [[4bt7|4bt7]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_decarboxylase Acetolactate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.5 4.1.1.5] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bt3 RCSB], [http://www.ebi.ac.uk/pdbsum/4bt3 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Acetolactate decarboxylase catalyzes the conversion of both enantiomers of acetolactate to the (R)-enantiomer of acetoin, via a mechanism that has been shown to involve a prior rearrangement of the non-natural (R)-enantiomer substrate to the natural (S)-enantiomer. In this paper, a series of crystal structures of ALDC complex with designed transition state mimics are reported. These structures, coupled with inhibition studies and site-directed mutagenesis provide an improved understanding of the molecular processes involved in the stereoselective decarboxylation/protonation events. A mechanism for the transformation of each enantiomer of acetolactate is proposed.
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==About this Structure==
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Structure and Mechanism of Acetolactate Decarboxylase.,Marlow VA, Rea D, Najmudin S, Wills M, Fulop V ACS Chem Biol. 2013 Aug 28. PMID:23985082<ref>PMID:23985082</ref>
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[[4bt3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023985082</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Acetolactate decarboxylase]]
[[Category: Acetolactate decarboxylase]]
[[Category: Brevibacillus brevis]]
[[Category: Brevibacillus brevis]]
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[[Category: Fulop, V.]]
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[[Category: Fulop, V]]
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[[Category: Marlow, V A.]]
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[[Category: Marlow, V A]]
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[[Category: Najmudin, S.]]
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[[Category: Najmudin, S]]
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[[Category: Rea, D.]]
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[[Category: Rea, D]]
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[[Category: Wills, M.]]
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[[Category: Wills, M]]
[[Category: Acetoin biosynthesis]]
[[Category: Acetoin biosynthesis]]
[[Category: Bifunctional enzyme]]
[[Category: Bifunctional enzyme]]
[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Stereoselective decarboxylation]]
[[Category: Stereoselective decarboxylation]]

Revision as of 09:11, 21 December 2014

acetolactate decarboxylase with a bound (2R,3R)-2,3-Dihydroxy-2- methylbutanoic acid

4bt3, resolution 1.10Å

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