1rjl

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[[Image:1rjl.gif|left|200px]]<br /><applet load="1rjl" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1rjl.gif|left|200px]]
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caption="1rjl, resolution 2.60&Aring;" />
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'''Structure of the complex between OspB-CT and bactericidal Fab-H6831'''<br />
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{{Structure
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|PDB= 1rjl |SIZE=350|CAPTION= <scene name='initialview01'>1rjl</scene>, resolution 2.60&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= OSPB, BBA16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=139 Borrelia burgdorferi])
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}}
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'''Structure of the complex between OspB-CT and bactericidal Fab-H6831'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1RJL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJL OCA].
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1RJL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJL OCA].
==Reference==
==Reference==
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Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target., Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL, J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15713683 15713683]
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Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target., Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL, J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713683 15713683]
[[Category: Borrelia burgdorferi]]
[[Category: Borrelia burgdorferi]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: beta sheet]]
[[Category: beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:52:27 2008''

Revision as of 11:52, 20 March 2008


PDB ID 1rjl

Drag the structure with the mouse to rotate
, resolution 2.60Å
Gene: OSPB, BBA16 (Borrelia burgdorferi)
Coordinates: save as pdb, mmCIF, xml



Structure of the complex between OspB-CT and bactericidal Fab-H6831


Overview

Certain antibody Fab fragments directed against the C terminus of outer surface protein B (OspB), a major lipoprotein of the Lyme disease spirochete, Borrelia burgdorferi, have the unusual property of being bactericidal even in the absence of complement. We report here x-ray crystal structures of a C-terminal fragment of B. burgdorferi OspB, which spans residues 152-296, alone at 2.0-A resolution, and in a complex with the bactericidal Fab H6831 at 2.6-A resolution. The H6831 epitope is topologically analogous to the LA-2 epitope of OspA and is centered around OspB Lys-253, a residue essential for H6831 recognition. A beta-sheet present in the free OspB fragment is either disordered or removed by proteolysis in the H6831-bound complex. Other conformational changes between free and H6831-bound structures are minor and appear to be related to this loss. In both crystal structures, OspB C-terminal fragments form artificial dimers connected by intermolecular beta-sheets. OspB structure, stability, and possible mechanisms of killing by H6831 and other bactericidal Fabs are discussed in light of the structural data.

About this Structure

1RJL is a Single protein structure of sequence from Borrelia burgdorferi and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target., Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL, J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:15713683

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