1rk8
From Proteopedia
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| - | [[Image:1rk8.gif|left|200px]] | + | [[Image:1rk8.gif|left|200px]] |
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| - | '''Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex''' | + | {{Structure |
| + | |PDB= 1rk8 |SIZE=350|CAPTION= <scene name='initialview01'>1rk8</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= tsu ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]), MAGO,MGN,CG9401 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]), WIBG,PYM,CG30176/CG10330 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RK8 is a [ | + | 1RK8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK8 OCA]. |
==Reference== | ==Reference== | ||
| - | Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex., Bono F, Ebert J, Unterholzner L, Guttler T, Izaurralde E, Conti E, EMBO Rep. 2004 Mar;5(3):304-10. Epub 2004 Feb 13. PMID:[http:// | + | Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex., Bono F, Ebert J, Unterholzner L, Guttler T, Izaurralde E, Conti E, EMBO Rep. 2004 Mar;5(3):304-10. Epub 2004 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14968132 14968132] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: mrna processing; rrm; rbd; nmd; oskar mrna localization]] | [[Category: mrna processing; rrm; rbd; nmd; oskar mrna localization]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:52:44 2008'' |
Revision as of 11:52, 20 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Gene: | tsu (Drosophila melanogaster), MAGO,MGN,CG9401 (Drosophila melanogaster), WIBG,PYM,CG30176/CG10330 (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex
Overview
The exon junction complex (EJC) is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. The Mago-Y14 heterodimer is a core component of the EJC. Recently, the protein PYM has been identified as an interacting partner of Mago-Y14. Here we show that PYM is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. PYM interacts directly with Mago-Y14 by means of its N-terminal domain. The crystal structure of the Drosophila ternary complex at 1.9 A resolution reveals that PYM binds Mago and Y14 simultaneously, capping their heterodimerization interface at conserved surface residues. Formation of this ternary complex is also observed with the human proteins. Mago residues involved in the interaction with PYM have been implicated in nonsense-mediated mRNA decay (NMD). Consistently, human PYM is active in NMD tethering assays. Together, these data suggest a role for PYM in NMD.
About this Structure
1RK8 is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex., Bono F, Ebert J, Unterholzner L, Guttler T, Izaurralde E, Conti E, EMBO Rep. 2004 Mar;5(3):304-10. Epub 2004 Feb 13. PMID:14968132
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