1rl1
From Proteopedia
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| - | [[Image:1rl1.gif|left|200px]] | + | [[Image:1rl1.gif|left|200px]] |
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| - | '''Solution structure of human Sgt1 CS domain''' | + | {{Structure |
| + | |PDB= 1rl1 |SIZE=350|CAPTION= <scene name='initialview01'>1rl1</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SUGT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of human Sgt1 CS domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RL1 is a [ | + | 1RL1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA]. |
==Reference== | ==Reference== | ||
| - | Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain., Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ, J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:[http:// | + | Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain., Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ, J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14761955 14761955] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Michowski, W.]] | [[Category: Michowski, W.]] | ||
[[Category: Nowotny, M.]] | [[Category: Nowotny, M.]] | ||
| - | [[Category: 7 beta | + | [[Category: 7 beta strand]] |
[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
[[Category: lacking last beta strand seen in p23]] | [[Category: lacking last beta strand seen in p23]] | ||
[[Category: similar to p23]] | [[Category: similar to p23]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:53:02 2008'' |
Revision as of 11:53, 20 March 2008
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| Gene: | SUGT1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of human Sgt1 CS domain
Overview
Sgt1 has been identified as a subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes and is also implicated in plant disease resistance. Sgt1 has two putative HSP90 binding domains, a tetratricopeptide repeat and a p23-like CHORD and Sgt1 (CS) domain. Using NMR spectroscopy, we show that only the CS domain of human Sgt1 physically interacts with HSP90. The tetratricopeptide repeat domain does not bind to either HSP90 or HSP70. Determination of the three-dimensional structure showed that the Sgt1-CS domain shares the same beta-sandwich fold as p23 but lacks the last highly conserved beta-strand in p23. Analysis of the structures of Sgt1-CS and p23 revealed a similar charge distribution on one of two opposing surfaces that suggests that it is the binding region for HSP90 in Sgt1. Although ATP is absolutely required for p23 binding to HSP90, Sgt1 binds to HSP90 also in the absence of the non-hydrolyzable analog ATPgammaS. Our findings suggest the CS domain is a binding module for HSP90 distinct from p23-like domains, which implies that Sgt1 and related proteins function in recruiting heat shock protein activities to multiprotein assemblies.
About this Structure
1RL1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain., Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ, J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:14761955
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