1ro0
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1ro0.gif|left|200px]] | + | [[Image:1ro0.gif|left|200px]] |
| - | + | ||
| - | '''Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M SeMet remote''' | + | {{Structure |
| + | |PDB= 1ro0 |SIZE=350|CAPTION= <scene name='initialview01'>1ro0</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M SeMet remote''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1RO0 is a [ | + | 1RO0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_islandicus Sulfolobus islandicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RO0 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a bifunctional DNA primase-polymerase., Lipps G, Weinzierl AO, von Scheven G, Buchen C, Cramer P, Nat Struct Mol Biol. 2004 Feb;11(2):157-62. Epub 2004 Jan 18. PMID:[http:// | + | Structure of a bifunctional DNA primase-polymerase., Lipps G, Weinzierl AO, von Scheven G, Buchen C, Cramer P, Nat Struct Mol Biol. 2004 Feb;11(2):157-62. Epub 2004 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14730355 14730355] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus islandicus]] | [[Category: Sulfolobus islandicus]] | ||
| Line 20: | Line 29: | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: dna polymerase]] | [[Category: dna polymerase]] | ||
| - | [[Category: evolution of nucleic acid polymerizing | + | [[Category: evolution of nucleic acid polymerizing enzyme]] |
[[Category: polymerization]] | [[Category: polymerization]] | ||
[[Category: primase]] | [[Category: primase]] | ||
[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:54:05 2008'' |
Revision as of 11:54, 20 March 2008
| |||||||
| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M SeMet remote
Overview
Genome replication generally requires primases, which synthesize an initial oligonucleotide primer, and DNA polymerases, which elongate the primer. Primase and DNA polymerase activities are combined, however, in newly identified replicases from archaeal plasmids, such as pRN1 from Sulfolobus islandicus. Here we present a structure-function analysis of the pRN1 primase-polymerase (prim-pol) domain. The crystal structure shows a central depression lined by conserved residues. Mutations on one side of the depression reduce DNA affinity. On the opposite side of the depression cluster three acidic residues and a histidine, which are required for primase and DNA polymerase activity. One acidic residue binds a manganese ion, suggestive of a metal-dependent catalytic mechanism. The structure does not show any similarity to DNA polymerases, but is distantly related to archaeal and eukaryotic primases, with corresponding active-site residues. We propose that archaeal and eukaryotic primases and the prim-pol domain have a common evolutionary ancestor, a bifunctional replicase for small DNA genomes.
About this Structure
1RO0 is a Single protein structure of sequence from Sulfolobus islandicus. Full crystallographic information is available from OCA.
Reference
Structure of a bifunctional DNA primase-polymerase., Lipps G, Weinzierl AO, von Scheven G, Buchen C, Cramer P, Nat Struct Mol Biol. 2004 Feb;11(2):157-62. Epub 2004 Jan 18. PMID:14730355
Page seeded by OCA on Thu Mar 20 13:54:05 2008
