3wcq
From Proteopedia
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| - | + | ==Crystal structure analysis of Cyanidioschyzon melorae ferredoxin D58N mutant== | |
| - | + | <StructureSection load='3wcq' size='340' side='right' caption='[[3wcq]], [[Resolution|resolution]] 0.97Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3wcq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyanidioschyzon_merolae Cyanidioschyzon merolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WCQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WCQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=45157 Cyanidioschyzon merolae])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wcq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wcq RCSB], [http://www.ebi.ac.uk/pdbsum/3wcq PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50 degrees C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97A) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP(+) reductase or cytochrome c. | ||
| - | + | Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation - A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant.,Ueno Y, Matsumoto T, Yamano A, Imai T, Morimoto Y Biochem Biophys Res Commun. 2013 Jul 12;436(4):736-9. doi:, 10.1016/j.bbrc.2013.06.029. Epub 2013 Jun 18. PMID:23792094<ref>PMID:23792094</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Ferredoxin|Ferredoxin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Cyanidioschyzon merolae]] | [[Category: Cyanidioschyzon merolae]] | ||
| - | [[Category: Imai, T | + | [[Category: Imai, T]] |
| - | [[Category: Matsumoto, T | + | [[Category: Matsumoto, T]] |
| - | [[Category: Morimoto, Y | + | [[Category: Morimoto, Y]] |
| - | [[Category: Ueno, Y | + | [[Category: Ueno, Y]] |
| - | [[Category: Yamano, A | + | [[Category: Yamano, A]] |
[[Category: 2fe-2s cluster]] | [[Category: 2fe-2s cluster]] | ||
[[Category: Electron transfer]] | [[Category: Electron transfer]] | ||
[[Category: Electron transport]] | [[Category: Electron transport]] | ||
[[Category: Fnr]] | [[Category: Fnr]] | ||
Revision as of 09:54, 21 December 2014
Crystal structure analysis of Cyanidioschyzon melorae ferredoxin D58N mutant
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