3zfh
From Proteopedia
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| - | + | ==Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase== | |
| - | + | <StructureSection load='3zfh' size='340' side='right' caption='[[3zfh]], [[Resolution|resolution]] 2.25Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3zfh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/IMP_dehydrogenase IMP dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.205 1.1.1.205] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zfh RCSB], [http://www.ebi.ac.uk/pdbsum/3zfh PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Inosine 5'-monophosphate dehydrogenase (IMPDH) represents a potential antimicrobial drug target. The crystal structure of recombinant Pseudomonas aeruginosa IMPDH has been determined to a resolution of 2.25 A. The structure is a homotetramer of subunits dominated by a (beta/alpha)8-barrel fold, consistent with other known structures of IMPDH. Also in common with previous work, the cystathionine beta-synthase domains, residues 92-204, are not present in the model owing to disorder. However, unlike the majority of available structures, clearly defined electron density exists for a loop that creates part of the active site. This loop, composed of residues 297-315, links alpha8 and beta9 and carries the catalytic Cys304. P. aeruginosa IMPDH shares a high level of sequence identity with bacterial and protozoan homologues, with residues involved in binding substrate and the NAD(+) cofactor being conserved. Specific differences that have been proven to contribute to selectivity against the human enzyme in a study of Cryptosporidium parvum IMPDH are also conserved, highlighting the potential value of IMPDH as a drug target. | ||
| - | + | Structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase.,Rao VA, Shepherd SM, Owen R, Hunter WN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):243-7. doi:, 10.1107/S1744309113002352. Epub 2013 Feb 22. PMID:23519796<ref>PMID:23519796</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | [[ | + | </div> |
| + | |||
| + | ==See Also== | ||
| + | *[[Inosine monophosphate dehydrogenase|Inosine monophosphate dehydrogenase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: IMP dehydrogenase]] | [[Category: IMP dehydrogenase]] | ||
[[Category: Pseudomonas aeruginosa pao1]] | [[Category: Pseudomonas aeruginosa pao1]] | ||
| - | [[Category: Hunter, W N | + | [[Category: Hunter, W N]] |
| - | [[Category: Owen, R | + | [[Category: Owen, R]] |
| - | [[Category: Rao, V A | + | [[Category: Rao, V A]] |
| - | [[Category: Shepherd, S M | + | [[Category: Shepherd, S M]] |
[[Category: Guanine nucleotide biosynthesis]] | [[Category: Guanine nucleotide biosynthesis]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 09:55, 21 December 2014
Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase
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