4b8d
From Proteopedia
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| - | + | ==TENSEGRITY TRIANGLE FROM ENZYMATICALLY MANUFACTURED DNA== | |
| - | + | <StructureSection load='4b8d' size='340' side='right' caption='[[4b8d]], [[Resolution|resolution]] 4.79Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4b8d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B8D FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b8d RCSB], [http://www.ebi.ac.uk/pdbsum/4b8d PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Single-stranded oligonucleotides are important as research tools, as diagnostic probes, in gene therapy and in DNA nanotechnology. Oligonucleotides are typically produced via solid-phase synthesis, using polymer chemistries that are limited relative to what biological systems produce. The number of errors in synthetic DNA increases with oligonucleotide length, and the resulting diversity of sequences can be a problem. Here we present the 'monoclonal stoichiometric' (MOSIC) method for enzyme-mediated production of DNA oligonucleotides. We amplified oligonucleotides from clonal templates derived from single bacterial colonies and then digested cutter hairpins in the products, which released pools of oligonucleotides with precisely controlled relative stoichiometric ratios. We prepared 14-378-nucleotide MOSIC oligonucleotides either by in vitro rolling-circle amplification or by amplification of phagemid DNA in Escherichia coli. Analyses of the formation of a DNA crystal and folding of DNA nanostructures confirmed the scalability, purity and stoichiometry of the produced oligonucleotides. | ||
| - | + | Enzymatic production of 'monoclonal stoichiometric' single-stranded DNA oligonucleotides.,Ducani C, Kaul C, Moche M, Shih WM, Hogberg B Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2503. PMID:23727986<ref>PMID:23727986</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Synthetic construct]] | [[Category: Synthetic construct]] | ||
| - | [[Category: Ducani, C | + | [[Category: Ducani, C]] |
| - | [[Category: Hogberg, B | + | [[Category: Hogberg, B]] |
| - | [[Category: Kaul, C D | + | [[Category: Kaul, C D]] |
| - | [[Category: Moche, M | + | [[Category: Moche, M]] |
| - | [[Category: Shih, W M | + | [[Category: Shih, W M]] |
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Dna origami]] | [[Category: Dna origami]] | ||
[[Category: Rolling circle amplification]] | [[Category: Rolling circle amplification]] | ||
Revision as of 09:58, 21 December 2014
TENSEGRITY TRIANGLE FROM ENZYMATICALLY MANUFACTURED DNA
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