4bes

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4bes| PDB=4bes | SCENE= }}
+
==Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate and phosphocholine==
-
===Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate and phosphocholine===
+
<StructureSection load='4bes' size='340' side='right' caption='[[4bes]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_23572077}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4bes]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BES FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bep|4bep]], [[4ber|4ber]], [[4bet|4bet]]</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bes OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bes RCSB], [http://www.ebi.ac.uk/pdbsum/4bes PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The FIC motif and the eukaryotic-like ankyrin repeats are found in many bacterial type IV effectors, yet little is known about how these domains enable bacteria to modulate host cell functions. Bacterial FIC domains typically bind ATP and transfer adenosine monophosphate moiety onto target proteins. The ankyrin repeat-containing protein AnkX encoded by the intracellular pathogen Legionella pneumophila is unique in that its FIC domain binds to CDP-choline and transfers a phosphocholine residue onto proteins in the Rab1 GTPase family. By determining the structures of unbound AnkX and AnkX with bound CDP-choline, CMP/phosphocholine and CMP, we demonstrate that the orientation of substrate binding in relation to the catalytic FIC motif enables this protein to function as a phosphocholinating enzyme rather than a nucleotidyl transferase. Additionally, the structure reveals that the ankyrin repeats mediate scaffolding interactions that resemble those found in protein-protein interactions, but are unprecedented in intramolecular interactions. Together with phosphocholination experiments, our structures unify a general phosphoryl transferase mechanism common to all FIC enzymes that should be conserved from bacteria to human.
-
==Function==
+
Structure of the Legionella effector AnkX reveals the mechanism of phosphocholine transfer by the FIC domain.,Campanacci V, Mukherjee S, Roy CR, Cherfils J EMBO J. 2013 Apr 9. doi: 10.1038/emboj.2013.82. PMID:23572077<ref>PMID:23572077</ref>
-
[[http://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH]] Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.<ref>PMID:18566289</ref> <ref>PMID:22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref> <ref>PMID:22411835</ref>
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[4bes]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BES OCA].
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:023572077</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]
[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]
-
[[Category: Campanacci, V.]]
+
[[Category: Campanacci, V]]
-
[[Category: Cherfils, J.]]
+
[[Category: Cherfils, J]]
-
[[Category: Mukherjee, S.]]
+
[[Category: Mukherjee, S]]
-
[[Category: Roy, C R.]]
+
[[Category: Roy, C R]]
[[Category: Phosphocholination]]
[[Category: Phosphocholination]]
[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Type iv secretion system effector]]
[[Category: Type iv secretion system effector]]

Revision as of 10:07, 21 December 2014

Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate and phosphocholine

4bes, resolution 2.54Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bes"
Personal tools