4e68
From Proteopedia
(Difference between revisions)
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| - | + | ==Unphosphorylated STAT3B core protein binding to dsDNA== | |
| - | === | + | <StructureSection load='4e68' size='340' side='right' caption='[[4e68]], [[Resolution|resolution]] 2.58Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4e68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E68 FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bg1|1bg1]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Aprf, Stat3, STAT3B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e68 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e68 RCSB], [http://www.ebi.ac.uk/pdbsum/4e68 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The STAT3 transcription factor plays a central role in a wide range of cancer types where it is over-expressed. Previously, phosphorylation of this protein was thought to be a prerequisite for direct binding to DNA. However, we have now shown complete binding of a purified unphosphorylated STAT3 (uSTAT3) core directly to M67 DNA, the high affinity STAT3 target DNA sequence, by a protein electrophoretic mobility shift assay (PEMSA). Binding to M67 DNA was inhibited by addition of increasing concentrations of a phosphotyrosyl peptide. X-ray crystallography demonstrates one mode of binding that is similar to that known for the STAT3 core phosphorylated at Y705. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: pSTAT3betatcandpSTAT3betatcbindbymolecular sieving(View interaction). | ||
| - | + | Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography.,Nkansah E, Shah R, Collie GW, Parkinson GN, Palmer J, Rahman KM, Bui TT, Drake AF, Husby J, Neidle S, Zinzalla G, Thurston DE, Wilderspin AF FEBS Lett. 2013 Feb 19. pii: S0014-5793(13)00110-5. doi:, 10.1016/j.febslet.2013.01.065. PMID:23434585<ref>PMID:23434585</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | <references | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
| - | [[Category: Collie, G W | + | [[Category: Collie, G W]] |
| - | [[Category: Parkinson, G N | + | [[Category: Parkinson, G N]] |
| - | [[Category: Shah, R | + | [[Category: Shah, R]] |
[[Category: Activator of transcription]] | [[Category: Activator of transcription]] | ||
[[Category: Cytosol]] | [[Category: Cytosol]] | ||
Revision as of 10:44, 21 December 2014
Unphosphorylated STAT3B core protein binding to dsDNA
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