4fwt
From Proteopedia
(Difference between revisions)
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| - | + | ==Complex structure of viral RNA polymerase form III== | |
| - | + | <StructureSection load='4fwt' size='340' side='right' caption='[[4fwt]], [[Resolution|resolution]] 3.20Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4fwt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FWT FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3agp|3agp]], [[3agq|3agq]], [[3vnu|3vnu]], [[3vnv|3vnv]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fwt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fwt RCSB], [http://www.ebi.ac.uk/pdbsum/4fwt PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The genomic RNA of Qbeta virus is replicated by Qbeta replicase, a template-dependent RNA polymerase complex. Qbeta replicase has an intrinsic template-independent RNA 3'-adenylation activity, which is required for efficient viral RNA amplification in the host cells. However, the mechanism of the template-independent 3'-adenylation of RNAs by Qbeta replicase has remained elusive. We determined the structure of a complex that includes Qbeta replicase, a template RNA, a growing RNA complementary to the template RNA, and ATP. The structure represents the terminal stage of RNA polymerization and reveals that the shape and size of the nucleotide-binding pocket becomes available for ATP accommodation after the 3'-penultimate template-dependent C-addition. The stacking interaction between the ATP and the neighboring Watson-Crick base pair, between the 5'-G in the template and the 3'-C in the growing RNA, contributes to the nucleotide specificity. Thus, the template for the template-independent 3'-adenylation by Qbeta replicase is the RNA and protein ribonucleoprotein complex. | ||
| - | + | Mechanism for template-independent terminal adenylation activity of Qbeta replicase.,Takeshita D, Yamashita S, Tomita K Structure. 2012 Oct 10;20(10):1661-9. doi: 10.1016/j.str.2012.07.004. Epub 2012, Aug 9. PMID:22884418<ref>PMID:22884418</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Synthetic construct]] | [[Category: Synthetic construct]] | ||
| - | [[Category: Takeshita, D | + | [[Category: Takeshita, D]] |
| - | [[Category: Tomita, K | + | [[Category: Tomita, K]] |
[[Category: Rna polymerase]] | [[Category: Rna polymerase]] | ||
[[Category: Transferase-rna complex]] | [[Category: Transferase-rna complex]] | ||
[[Category: Translation]] | [[Category: Translation]] | ||
Revision as of 10:48, 21 December 2014
Complex structure of viral RNA polymerase form III
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