1rvj
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1rvj.gif|left|200px]] | + | [[Image:1rvj.gif|left|200px]] |
| - | + | ||
| - | '''PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG H177 REPLACED WITH HIS''' | + | {{Structure |
| + | |PDB= 1rvj |SIZE=350|CAPTION= <scene name='initialview01'>1rvj</scene>, resolution 2.75Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene>, <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene> and <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= PUFL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), PUFM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), PUHA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]) | ||
| + | }} | ||
| + | |||
| + | '''PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG H177 REPLACED WITH HIS''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1RVJ is a [ | + | 1RVJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RVJ OCA]. |
==Reference== | ==Reference== | ||
| - | X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:[http:// | + | X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15062092 15062092] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rhodobacter sphaeroides]] | [[Category: Rhodobacter sphaeroides]] | ||
| Line 32: | Line 41: | ||
[[Category: proton transfer pathway]] | [[Category: proton transfer pathway]] | ||
[[Category: revertant]] | [[Category: revertant]] | ||
| - | [[Category: rhodobacter | + | [[Category: rhodobacter sphaeroide]] |
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:56:49 2008'' |
Revision as of 11:56, 20 March 2008
| |||||||
| , resolution 2.75Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , , and | ||||||
| Gene: | PUFL (Rhodobacter sphaeroides), PUFM (Rhodobacter sphaeroides), PUHA (Rhodobacter sphaeroides) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG H177 REPLACED WITH HIS
Overview
In the photosynthetic reaction center (RC) from Rhodobacter sphaeroides, the reduction of a bound quinone molecule Q(B) is coupled with proton uptake. When Asp-L213 is replaced by Asn, proton transfer is inhibited. Proton transfer was restored by two second-site revertant mutations, Arg-M233-->Cys and Arg-H177-->His. Kinetic effects of Cd(2+) on proton transfer showed that the entry point in revertant RCs to be the same as in the native RC. The structures of the parental and two revertant RCs were determined at resolutions of 2.10, 1.80, and 2.75 A. From the structures, we were able to delineate alternate proton transfer pathways in the revertants. The main changes occur near Glu-H173, which allow it to substitute for the missing Asp-L213. The electrostatic changes near Glu-H173 cause it to be a good proton donor and acceptor, and the structural changes create a cavity which accommodates water molecules that connect Glu-H173 to other proton transfer components.
About this Structure
1RVJ is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.
Reference
X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:15062092
Page seeded by OCA on Thu Mar 20 13:56:49 2008
Categories: Protein complex | Rhodobacter sphaeroides | Abresch, E C. | Axelrod, H L. | Feher, G. | Okamura, M Y. | Paddock, M L. | Xu, Q. | BCL | BPB | BPH | CDL | FE2 | LDA | PO4 | SPO | U10 | Bacterial photosynthesis | Integral membrane protein | Proton transfer pathway | Revertant | Rhodobacter sphaeroide | X-ray crystallography
