4h02
From Proteopedia
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| - | + | ==Crystal structure of P. falciparum Lysyl-tRNA synthetase== | |
| - | === | + | <StructureSection load='4h02' size='340' side='right' caption='[[4h02]], [[Resolution|resolution]] 2.91Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4h02]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H02 FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bju|3bju]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF13_0262 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 Plasmodium falciparum 3D7])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h02 RCSB], [http://www.ebi.ac.uk/pdbsum/4h02 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aminoacyl-tRNA synthetases are essential enzymes that transmit information from the genetic code to proteins in cells and are targets for antipathogen drug development. Elucidation of the crystal structure of cytoplasmic lysyl-tRNA synthetase from the malaria parasite Plasmodium falciparum (PfLysRS) has allowed direct comparison with human LysRS. The authors' data suggest that PfLysRS is dimeric in solution, whereas the human counterpart can also adopt tetrameric forms. It is shown for the first time that PfLysRS is capable of synthesizing the signalling molecule Ap4a (diadenosine tetraphosphate) using ATP as a substrate. The PfLysRS crystal structure is in the apo form, such that binding to ATP will require rotameric changes in four conserved residues. Differences in the active-site regions of parasite and human LysRSs suggest the possibility of exploiting PfLysRS for selective inhibition. These investigations on PfLysRS further validate malarial LysRSs as attractive antimalarial targets and provide new structural space for the development of inhibitors that target pathogen LysRSs selectively. | ||
| - | + | Structural analysis of malaria-parasite lysyl-tRNA synthetase provides a platform for drug development.,Khan S, Garg A, Camacho N, Van Rooyen J, Kumar Pole A, Belrhali H, Ribas de Pouplana L, Sharma V, Sharma A Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):785-95. doi:, 10.1107/S0907444913001923. Epub 2013 Apr 11. PMID:23633587<ref>PMID:23633587</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Lysine--tRNA ligase]] | [[Category: Lysine--tRNA ligase]] | ||
[[Category: Plasmodium falciparum 3d7]] | [[Category: Plasmodium falciparum 3d7]] | ||
| - | [[Category: Garg, A | + | [[Category: Garg, A]] |
| - | [[Category: Khan, S | + | [[Category: Khan, S]] |
| - | [[Category: Sharma, A | + | [[Category: Sharma, A]] |
[[Category: Cladosporin]] | [[Category: Cladosporin]] | ||
[[Category: Ligase]] | [[Category: Ligase]] | ||
[[Category: Malaria]] | [[Category: Malaria]] | ||
Revision as of 11:14, 21 December 2014
Crystal structure of P. falciparum Lysyl-tRNA synthetase
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