4eqq
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Structure of Ltp, a superinfection exclusion protein from the Streptococcus thermophilus temperate phage TP-J34== | |
| - | + | <StructureSection load='4eqq' size='340' side='right' caption='[[4eqq]], [[Resolution|resolution]] 2.05Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4eqq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_phage_tp-j34 Streptococcus phage tp-j34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ltp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=73422 Streptococcus phage TP-J34])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eqq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eqq RCSB], [http://www.ebi.ac.uk/pdbsum/4eqq PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lipoproteins of temperate phage are a broad family of membrane proteins encoded in the lysogeny module of temperate phages. Expression of the ltpTP - J 34 gene of temperate Streptococcus thermophilus phage TP-J34 interferes with phage infection at the stage of triggering DNA release and injection into the cell. Here, we report the first structure of a superinfection exclusion protein. We have expressed and determined the X-ray structure of LtpTP - J 34 . The soluble domain of LtpTP - J 34 is composed of a tandem of three-helix helix-turn-helix (HTH) domains exhibiting a highly negatively charged surface. By isolating mutants of lactococcal phage P008wt with reduced sensitivities to LtpTP -J34 and by genome sequencing of such mutants we obtained evidence supporting the notion that LtpTP - J 34 targets the phage's tape measure protein (TMP) and blocks its insertion into the cytoplasmic membrane. | ||
| - | + | X-ray structure of a superinfection exclusion lipoprotein from phage TP-J34 and identification of the tape measure protein as its target.,Bebeacua C, Lorenzo Fajardo JC, Blangy S, Spinelli S, Bollmann S, Neve H, Cambillau C, Heller KJ Mol Microbiol. 2013 Jul;89(1):152-65. doi: 10.1111/mmi.12267. Epub 2013 Jun 7. PMID:23692331<ref>PMID:23692331</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Streptococcus phage tp-j34]] | [[Category: Streptococcus phage tp-j34]] | ||
| - | [[Category: Bebeacua, C | + | [[Category: Bebeacua, C]] |
| - | [[Category: Blangy, S | + | [[Category: Blangy, S]] |
| - | [[Category: Cambillau,C | + | [[Category: Cambillau,C]] |
| - | [[Category: Heller,K | + | [[Category: Heller,K]] |
| - | [[Category: Lorenzo, C | + | [[Category: Lorenzo, C]] |
| - | [[Category: Spinelli, S | + | [[Category: Spinelli, S]] |
[[Category: Hth fold]] | [[Category: Hth fold]] | ||
[[Category: Lipoprotein ektodomain]] | [[Category: Lipoprotein ektodomain]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Superinfection exclusion]] | [[Category: Superinfection exclusion]] | ||
Revision as of 11:30, 21 December 2014
Structure of Ltp, a superinfection exclusion protein from the Streptococcus thermophilus temperate phage TP-J34
| |||||||||||
