4h11
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Interaction partners of PSD-93 studied by X-ray crystallography and fluorescent polarization spectroscopy== | |
| - | + | <StructureSection load='4h11' size='340' side='right' caption='[[4h11]], [[Resolution|resolution]] 1.67Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4h11]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H11 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dlg2, Dlgh2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h11 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h11 RCSB], [http://www.ebi.ac.uk/pdbsum/4h11 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PSD-93 (chapsyn-110, DLG2) is a member of the family of membrane-associated guanylate kinase (MAGUK) proteins. The MAGUK proteins are involved in receptor localization and signalling pathways. The best characterized MAGUK protein, PSD-95, is known to be involved in NMDA receptor signalling via its PDZ domains. The PDZ domains of PSD-95 and PSD-93 are structurally very similar, but relatively little is known about the function of PSD-93. PSD-93 has been suggested to interact with GluD2 from the family of ionotropic glutamate receptors. Here, the interactions of four residues (GTSI) representing the extreme C-terminus of GluD2 with PSD-93 PDZ1 have been investigated in the crystalline phase. Two different binding modes of these residues were observed, suggesting that the peptide is not tightly bound to PSD-93 PDZ1. In accordance, the two N-terminal PSD-93 PDZ domains show no appreciable binding affinity for a GluD2-derived C-terminal octapeptide, whereas micromolar affinity was observed for a GluN2B-derived C-terminal octapeptide. This indicates that if present, the interactions between GluD2 and PSD-93 involve more than the extreme terminus of the receptor. In contrast, the tumour-suppressor protein SCRIB PDZ3 shows low micromolar affinity towards the GluD2-derived octapeptide, which is in agreement with previous findings using high-throughput assays. | ||
| - | + | Interaction partners of PSD-93 studied by X-ray crystallography and fluorescence polarization spectroscopy.,Fiorentini M, Bach A, Stromgaard K, Kastrup JS, Gajhede M Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):587-94. doi:, 10.1107/S0907444912051839. Epub 2013 Mar 14. PMID:23519667<ref>PMID:23519667</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
| - | [[Category: Fiorentini, M | + | [[Category: Fiorentini, M]] |
| - | [[Category: Gajhede, M | + | [[Category: Gajhede, M]] |
| - | [[Category: Kastrup, J S | + | [[Category: Kastrup, J S]] |
[[Category: All beta pdz]] | [[Category: All beta pdz]] | ||
[[Category: Neuropeptide]] | [[Category: Neuropeptide]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Protein interaction]] | [[Category: Protein interaction]] | ||
Revision as of 11:34, 21 December 2014
Interaction partners of PSD-93 studied by X-ray crystallography and fluorescent polarization spectroscopy
| |||||||||||
