1ryh
From Proteopedia
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| - | [[Image:1ryh.gif|left|200px]] | + | [[Image:1ryh.gif|left|200px]] |
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| - | '''Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase''' | + | {{Structure |
| + | |PDB= 1ryh |SIZE=350|CAPTION= <scene name='initialview01'>1ryh</scene>, resolution 1.75Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER'>GNP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RYH is a [ | + | 1RYH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYH OCA]. |
==Reference== | ==Reference== | ||
| - | Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase., Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR, J Biol Chem. 2004 Feb 6;279(6):4743-9. Epub 2003 Nov 18. PMID:[http:// | + | Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase., Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR, J Biol Chem. 2004 Feb 6;279(6):4743-9. Epub 2003 Nov 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14625275 14625275] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: gtp binding]] | [[Category: gtp binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:57:58 2008'' |
Revision as of 11:57, 20 March 2008
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| , resolution 1.75Å | |||||||
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| Ligands: | and | ||||||
| Activity: | Small monomeric GTPase, with EC number 3.6.5.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase
Overview
Rac1b was recently identified in malignant colorectal tumors as an alternative splice variant of Rac1 containing a 19-amino acid insertion next to the switch II region. The structures of Rac1b in the GDP- and the GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the insertion induces an open switch I conformation and a highly mobile switch II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. Interestingly, Rac1b is able to bind the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. The presented study provides insights into the structural and biochemical mechanism of a self-activating GTPase.
About this Structure
1RYH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase., Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR, J Biol Chem. 2004 Feb 6;279(6):4743-9. Epub 2003 Nov 18. PMID:14625275
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