4es4

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{{STRUCTURE_4es4| PDB=4es4 | SCENE= }}
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==Crystal structure of YdiV and FlhD complex==
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===Crystal structure of YdiV and FlhD complex===
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<StructureSection load='4es4' size='340' side='right' caption='[[4es4]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23002140}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4es4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ES4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ES4 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tlq|3tlq]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cdgR, ydiV, b1707, JW1697 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12]), flhD, flbB, b1892, JW1881 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4es4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4es4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4es4 RCSB], [http://www.ebi.ac.uk/pdbsum/4es4 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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YdiV is a negative regulator of cell motility. It interacts with FlhD(4)C(2) complex, a product of flagellar master operon, which works as the transcription activator of all other flagellar operons. Here, we report the crystal structures of YdiV and YdiV(2)-FlhD(2) complex at 1.9 A and 2.9 A resolutions, respectively. Interestingly, YdiV formed multiple types of complexes with FlhD(4)C(2). YdiV(1)-FlhD(4)C(2) and YdiV(2)-FlhD(4)C(2) still bound to DNA, while YdiV(3)-FlhD(4)C(2) and YdiV(4)-FlhD(4)C(2) did not. DNA bound FlhD(4)C(2) through wrapping around the FlhC subunit rather than the FlhD subunit. Structural analysis showed that only two peripheral FlhD subunits were accessible for YdiV binding, forming the YdiV(2)-FlhD(4)C(2) complex without affecting the integrity of ring-like structure. YdiV(2)-FlhD(2) structure and the negative staining electron microscopy reconstruction of YdiV(4)-FlhD(4)C(2) suggested that the third and fourth YdiV molecule bound to the FlhD(4)C(2) complex through squeezing into the ring-like structure of FlhD(4)C(2) between the two internal D subunits. Consequently, the ring-like structure opened up, and the complex lost DNA-binding ability. Thus, YdiV inhibits FlhD(4)C(2) only at relatively high concentrations.
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==Function==
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Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility.,Li B, Li N, Wang F, Guo L, Huang Y, Liu X, Wei T, Zhu D, Liu C, Pan H, Xu S, Wang HW, Gu L Nucleic Acids Res. 2012 Sep 21. PMID:23002140<ref>PMID:23002140</ref>
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[[http://www.uniprot.org/uniprot/YDIV_ECOLI YDIV_ECOLI]] Upon overexpression acts as a novel anti-FlhC(2)FlhD(4) factor, decreasing its DNA-binding activity, able to negatively regulate expression of flagellar class II operons including FliC.<ref>PMID:22461489</ref> [[http://www.uniprot.org/uniprot/FLHD_ECOLI FLHD_ECOLI]] Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.<ref>PMID:7961507</ref> <ref>PMID:11169100</ref> <ref>PMID:15941987</ref> <ref>PMID:18765794</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4es4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ES4 OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023002140</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
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[[Category: Gu, L.]]
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[[Category: Gu, L]]
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[[Category: Li, B.]]
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[[Category: Li, B]]
[[Category: Flagellar regulation]]
[[Category: Flagellar regulation]]
[[Category: Transcription]]
[[Category: Transcription]]

Revision as of 11:42, 21 December 2014

Crystal structure of YdiV and FlhD complex

4es4, resolution 2.90Å

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