1rz9
From Proteopedia
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| - | [[Image:1rz9.gif|left|200px]] | + | [[Image:1rz9.gif|left|200px]] |
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| - | '''Crystal Structure of AAV Rep complexed with the Rep-binding sequence''' | + | {{Structure |
| + | |PDB= 1rz9 |SIZE=350|CAPTION= <scene name='initialview01'>1rz9</scene>, resolution 3.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Adeno-associated virus - 5]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of AAV Rep complexed with the Rep-binding sequence''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RZ9 is a [ | + | 1RZ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. |
==Reference== | ==Reference== | ||
| - | The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces., Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F, Mol Cell. 2004 Feb 13;13(3):403-14. PMID:[http:// | + | The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces., Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F, Mol Cell. 2004 Feb 13;13(3):403-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14967147 14967147] |
[[Category: Adeno-associated virus - 5]] | [[Category: Adeno-associated virus - 5]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:58:17 2008'' |
Revision as of 11:58, 20 March 2008
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| , resolution 3.10Å | |||||||
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| Gene: | rep (Adeno-associated virus - 5) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of AAV Rep complexed with the Rep-binding sequence
Overview
Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, we have determined the structures of the Rep endonuclease domain separately complexed with two DNA substrates: the Rep binding site within the viral inverted terminal repeat and one of the terminal hairpin arms. At the Rep binding site, five Rep monomers bind five tetranucleotide direct repeats; each repeat is recognized by two Rep monomers from opposing faces of the DNA. Stem-loop binding involves a protein interface on the opposite side of the molecule from the active site where ssDNA is cleaved. Rep therefore has three distinct binding sites within its endonuclease domain for its different DNA substrates. Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration.
About this Structure
1RZ9 is a Single protein structure of sequence from Adeno-associated virus - 5. Full crystallographic information is available from OCA.
Reference
The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces., Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F, Mol Cell. 2004 Feb 13;13(3):403-14. PMID:14967147
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