4j7o
From Proteopedia
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| - | + | ==Structure of the N-terminal Repeat Domain of Rickettsia Sca2== | |
| - | + | <StructureSection load='4j7o' size='340' side='right' caption='[[4j7o]], [[Resolution|resolution]] 2.17Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4j7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rickettsia_conorii Rickettsia conorii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J7O FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sca2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=781 Rickettsia conorii])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j7o RCSB], [http://www.ebi.ac.uk/pdbsum/4j7o PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sca2 (surface cell antigen 2) is the only bacterial protein known to promote both actin filament nucleation and profilin-dependent elongation, mimicking eukaryotic formins to assemble actin comet tails for Rickettsia motility. We show that Sca2's functional mimicry of formins is achieved through a unique mechanism. Unlike formins, Sca2 is monomeric, but has N- and C-terminal repeat domains (NRD and CRD) that interact with each other for processive barbed-end elongation. The crystal structure of NRD reveals a previously undescribed fold, consisting of helix-loop-helix repeats arranged into an overall crescent shape. CRD is predicted to share this fold and might form together with NRD, a doughnut-shaped formin-like structure. In between NRD and CRD, proline-rich sequences mediate the incorporation of profilin-actin for elongation, and WASP-homology 2 (WH2) domains recruit actin monomers for nucleation. Sca2's alpha-helical fold is unusual among Gram-negative autotransporters, which overwhelmingly fold as beta-solenoids. Rickettsia has therefore "rediscovered" formin-like actin nucleation and elongation. | ||
| - | + | Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism.,Madasu Y, Suarez C, Kast DJ, Kovar DR, Dominguez R Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):E2677-86. doi:, 10.1073/pnas.1307235110. Epub 2013 Jul 1. PMID:23818602<ref>PMID:23818602</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Rickettsia conorii]] | [[Category: Rickettsia conorii]] | ||
| - | [[Category: Dominguez, R | + | [[Category: Dominguez, R]] |
| - | [[Category: Madasu, Y | + | [[Category: Madasu, Y]] |
[[Category: Actin]] | [[Category: Actin]] | ||
[[Category: Actin nucleation]] | [[Category: Actin nucleation]] | ||
[[Category: Cell invasion]] | [[Category: Cell invasion]] | ||
[[Category: Helical repeat]] | [[Category: Helical repeat]] | ||
Revision as of 12:02, 21 December 2014
Structure of the N-terminal Repeat Domain of Rickettsia Sca2
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