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4jbu
From Proteopedia
(Difference between revisions)
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| - | + | ==1.65A structure of the T3SS tip protein LcrV (G28-D322, C273S) from Yersinia pestis== | |
| - | + | <StructureSection load='4jbu' size='340' side='right' caption='[[4jbu]], [[Resolution|resolution]] 1.65Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4jbu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JBU FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jbu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jbu RCSB], [http://www.ebi.ac.uk/pdbsum/4jbu PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The human pathogen Yersinia pestis requires the assembly of the type III secretion system (T3SS) for virulence. The structural component of the T3SS contains an external needle and a tip complex, which is formed by LcrV in Y. pestis. The structure of an LcrV triple mutant (K40A/D41A/K42A) in a C273S background has previously been reported to 2.2 A resolution. Here, the crystal structure of LcrV without the triple mutation in a C273S background is reported at a higher resolution of 1.65 A. Overall the two structures are similar, but there are also notable differences, particularly near the site of the triple mutation. The refined structure revealed a slight shift in the backbone positions of residues Gly28-Asn43 and displayed electron density in the loop region consisting of residues Ile46-Val63, which was disordered in the original structure. In addition, the helical turn region spanning residues Tyr77-Gln95 adopts a different orientation. | ||
| - | + | Structure of the Yersinia pestis tip protein LcrV refined to 1.65 A resolution.,Chaudhury S, Battaile KP, Lovell S, Plano GV, De Guzman RN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May;69(Pt 5):477-81. doi: , 10.1107/S1744309113008579. Epub 2013 Apr 27. PMID:23695558<ref>PMID:23695558</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Yersinia pestis]] | [[Category: Yersinia pestis]] | ||
| - | [[Category: Battaile, K P | + | [[Category: Battaile, K P]] |
| - | [[Category: Chaudhury, S | + | [[Category: Chaudhury, S]] |
| - | [[Category: Guzman, R N.De | + | [[Category: Guzman, R N.De]] |
| - | [[Category: Lovell, S | + | [[Category: Lovell, S]] |
| - | [[Category: Plano, G | + | [[Category: Plano, G]] |
[[Category: Lcrv]] | [[Category: Lcrv]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
Revision as of 12:08, 21 December 2014
1.65A structure of the T3SS tip protein LcrV (G28-D322, C273S) from Yersinia pestis
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