4jas

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{{STRUCTURE_4jas| PDB=4jas | SCENE= }}
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==Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)==
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===Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)===
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<StructureSection load='4jas' size='340' side='right' caption='[[4jas]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23954504}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jas]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima_msb8 Thermotoga maritima msb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JAS FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BFD:ASPARTATE+BERYLLIUM+TRIFLUORIDE'>BFD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ja2|4ja2]], [[4jau|4jau]], [[4jav|4jav]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TM_0853 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 Thermotoga maritima MSB8]), TM_0468 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 Thermotoga maritima MSB8])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jas OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jas RCSB], [http://www.ebi.ac.uk/pdbsum/4jas PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two-component signal transduction systems typically involve a sensor histidine kinase that specifically phosphorylates a single, cognate response regulator. This protein-protein interaction relies on molecular recognition via a small set of residues in each protein. To better understand how these residues determine the specificity of kinase-substrate interactions, we rationally rewired the interaction interface of a Thermotoga maritima two-component system, HK853-RR468, to match that found in a different two-component system, Escherichia coli PhoR-PhoB. The rewired proteins interacted robustly with each other, but no longer interacted with the parent proteins. Analysis of the crystal structures of the wild-type and mutant protein complexes and a systematic mutagenesis study reveal how individual mutations contribute to the rewiring of interaction specificity. Our approach and conclusions have implications for studies of other protein-protein interactions and protein evolution and for the design of novel protein interfaces.
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==About this Structure==
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Structural Basis of a Rationally Rewired Protein-Protein Interface Critical to Bacterial Signaling.,Podgornaia AI, Casino P, Marina A, Laub MT Structure. 2013 Aug 13. pii: S0969-2126(13)00254-2. doi:, 10.1016/j.str.2013.07.005. PMID:23954504<ref>PMID:23954504</ref>
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[[4jas]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima_msb8 Thermotoga maritima msb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023954504</ref><references group="xtra"/><references/>
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</div>
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==See Also==
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*[[Response regulator|Response regulator]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Histidine kinase]]
[[Category: Histidine kinase]]
[[Category: Thermotoga maritima msb8]]
[[Category: Thermotoga maritima msb8]]
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[[Category: Casino, P.]]
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[[Category: Casino, P]]
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[[Category: Laub, M T.]]
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[[Category: Laub, M T]]
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[[Category: Marina, A.]]
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[[Category: Marina, A]]
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[[Category: Podgornaia, A I.]]
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[[Category: Podgornaia, A I]]
[[Category: Alpha/beta domain]]
[[Category: Alpha/beta domain]]
[[Category: Autophosphorylation]]
[[Category: Autophosphorylation]]
[[Category: Bergerat fold]]
[[Category: Bergerat fold]]
[[Category: Dephosphorylation]]
[[Category: Dephosphorylation]]
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[[Category: Histidine kinase]]
 
[[Category: Phosphorylation]]
[[Category: Phosphorylation]]
[[Category: Phosphotransferase]]
[[Category: Phosphotransferase]]

Revision as of 12:09, 21 December 2014

Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)

4jas, resolution 3.00Å

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