4he7
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Crystal Structure of Brazzein== | |
| - | === | + | <StructureSection load='4he7' size='340' side='right' caption='[[4he7]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4he7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pentadiplandra_brazzeana Pentadiplandra brazzeana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HE7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4he7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4he7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4he7 RCSB], [http://www.ebi.ac.uk/pdbsum/4he7 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Brazzein is the smallest sweet-tasting protein and was isolated from the wild African plant Pentadiplandra brazzeana. The brazzein molecule consists of 54 amino-acid residues and four disulfide bonds. Here, the first crystal structure of brazzein is reported at 1.8 A resolution and is compared with previously reported solution structures. Despite the overall structural similarity, there are several remarkable differences between the crystal and solution structures both in their backbone folds and side-chain conformations. Firstly, there is an additional alpha-helix in the crystal structure. Secondly, the atomic r.m.s.d.s between the corresponding C(alpha)-atom pairs are as large as 2.0-2.2 A between the crystal and solution structures. Thirdly, the crystal structure exhibits a molecular shape that is similar but not identical to the solution structures. The crystal structure of brazzein reported here will provide additional information and further insights into the intermolecular interaction of brazzein with the sweet-taste receptor. | ||
| - | + | The structure of brazzein, a sweet-tasting protein from the wild African plant Pentadiplandra brazzeana.,Nagata K, Hongo N, Kameda Y, Yamamura A, Sasaki H, Lee WC, Ishikawa K, Suzuki E, Tanokura M Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):642-7. doi:, 10.1107/S0907444913001005. Epub 2013 Mar 14. PMID:23519673<ref>PMID:23519673</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Pentadiplandra brazzeana]] | [[Category: Pentadiplandra brazzeana]] | ||
| - | [[Category: Hongo, N | + | [[Category: Hongo, N]] |
| - | [[Category: Ishikawa, K | + | [[Category: Ishikawa, K]] |
| - | [[Category: Kameda, Y | + | [[Category: Kameda, Y]] |
| - | [[Category: Lee, W C | + | [[Category: Lee, W C]] |
| - | [[Category: Nagata, K | + | [[Category: Nagata, K]] |
| - | [[Category: Sasaki, H | + | [[Category: Sasaki, H]] |
| - | [[Category: Suzuki, E | + | [[Category: Suzuki, E]] |
| - | [[Category: Tanokura, M | + | [[Category: Tanokura, M]] |
| - | [[Category: Yamamura, A | + | [[Category: Yamamura, A]] |
[[Category: Plant protein]] | [[Category: Plant protein]] | ||
[[Category: Sweet-tasting protein]] | [[Category: Sweet-tasting protein]] | ||
Revision as of 12:10, 21 December 2014
Crystal Structure of Brazzein
| |||||||||||
