1s23
From Proteopedia
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| - | [[Image:1s23.gif|left|200px]] | + | [[Image:1s23.gif|left|200px]] |
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| - | '''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG''' | + | {{Structure |
| + | |PDB= 1s23 |SIZE=350|CAPTION= <scene name='initialview01'>1s23</scene>, resolution 1.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S23 is a [ | + | 1S23 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA]. |
==Reference== | ==Reference== | ||
| - | DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:[http:// | + | DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15039556 15039556] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Murshudov, G N.]] | [[Category: Murshudov, G N.]] | ||
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[[Category: variability]] | [[Category: variability]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:59:25 2008'' |
Revision as of 11:59, 20 March 2008
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| , resolution 1.60Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG
Overview
The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in four different space groups. The crystals diffract to moderate resolution (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher resolution (1.6 A) is presented which was obtained thanks to the use of Co2+ and cryogenic temperatures. The availability of five different crystal structures allows a thorough analysis of the conformational variability of this DNA sequence. It is concluded that the central hexamer sequence CAATTG has a practically constant conformation under all conditions, whilst the terminal base pairs at both ends vary considerably as a result of differing interactions in the crystals. The new crystal structure presented here is stabilized by guanine-Co2+-guanine interactions and the formation of C1+ -G8.C3 triplexes between neighbouring duplexes. As a result of the higher resolution of the crystal structure, a more regular structure was obtained and a clear definition of the spine of hydration was observed which was not visible in the four previous structures.
About this Structure
1S23 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:15039556
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