4i60
From Proteopedia
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| - | + | ==Crystal structure of avidin - biotinylruthenocene complex== | |
| - | + | <StructureSection load='4i60' size='340' side='right' caption='[[4i60]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | { | + | == Structural highlights == |
| + | <table><tr><td colspan='2'>[[4i60]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I60 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B1R:[(1,2,3,4,5-ETA)-CYCLOPENTADIENYL][(1,2,3,4,5-ETA)-{5-[(3AS,4S,6AR)-2-OXOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYL}CYCLOPENTADIENYL]RUTHENIUM'>B1R</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1avd|1avd]], [[2avi|2avi]], [[1ave|1ave]], [[1vyo|1vyo]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i60 RCSB], [http://www.ebi.ac.uk/pdbsum/4i60 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of avidin, a protein from hen egg white, was determined in the form of a complex with biotinylruthenocene. This biotin-derived organometallic ligand is a potential anticancer agent for targeted therapy based upon avidin-biotin technology. Isothermal titration calorimetry experiments, involving avidin complexes with biotin (vitamin H or B7) derivatives, show differences in their affinity to the protein in comparison to its avidin-biotin complex, the strongest known biochemical interaction in Nature. The crystal structure of the first complex of avidin with biotinylruthenocene, determined at 2.5A resolution (PDB: 4I60), shows unique interactions of the ruthenocene moiety with avidin. Biotin derivatives exhibit weaker affinity to avidin then biotin, which allows their wider use in biotechnology. The specific properties of biotinylruthenocene and the knowledge of its interactions with avidin may be useful in biochemical, medical, and nanotechnological applications. | ||
| - | + | Structural investigation of the interactions of biotinylruthenocene with avidin.,Strzelczyk P, Bujacz A, Plazuk D, Zakrzewski J, Bujacz G Chem Biol Interact. 2013 Jun 25;204(1):6-12. doi: 10.1016/j.cbi.2013.04.005. Epub, 2013 Apr 18. PMID:23603015<ref>PMID:23603015</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Avidin|Avidin]] | |
| - | [[Category: | + | == References == |
| - | [[Category: Bujacz, A | + | <references/> |
| - | [[Category: Bujacz, G | + | __TOC__ |
| - | [[Category: Strzelczyk, P | + | </StructureSection> |
| + | [[Category: Chick]] | ||
| + | [[Category: Bujacz, A]] | ||
| + | [[Category: Bujacz, G]] | ||
| + | [[Category: Strzelczyk, P]] | ||
[[Category: Beta barrel]] | [[Category: Beta barrel]] | ||
[[Category: Biotin-binding protein]] | [[Category: Biotin-binding protein]] | ||
Revision as of 12:13, 21 December 2014
Crystal structure of avidin - biotinylruthenocene complex
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