4iqj

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{{Large structure}}
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==Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome==
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{{STRUCTURE_4iqj| PDB=4iqj | SCENE= }}
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<StructureSection load='4iqj' size='340' side='right' caption='[[4iqj]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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===Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome===
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== Structural highlights ==
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{{ABSTRACT_PUBMED_23478062}}
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<table><tr><td colspan='2'>[[4iqj]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IQJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2hpi|2hpi]], [[3e0d|3e0d]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iqj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iqj RCSB], [http://www.ebi.ac.uk/pdbsum/4iqj PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The C-terminal domain (CTD) of the tau subunit of the clamp loader (tauc) binds to both the DnaB helicase and the DNA polymerase III alpha subunit (PolIIIalpha), and determines their relative positions and orientations on the leading and lagging strands. Here, we present a 3.2 A resolution structure of Thermus aquaticus PolIIIalpha in complex with tauc and a DNA substrate. The structure reveals that the CTD of tauc interacts with the CTD of PolIIIalpha through its C-terminal helix and the adjacent loop. Additionally, in this complex PolIIIalpha displays an open conformation that includes the reorientations of the oligonucleotide-binding fold and the thumb domain, which may be an indirect result of crystal packing due to the presence of the tauc. Nevertheless, the position of the tauc on PolIIIalpha allows us to suggest an approximate model for how the PolIIIalpha is oriented and positioned on the DnaB helicase.
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==Function==
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Structure of the PolIIIalpha-tauc-DNA complex suggests an atomic model of the replisome.,Liu B, Lin J, Steitz TA Structure. 2013 Apr 2;21(4):658-64. doi: 10.1016/j.str.2013.02.002. Epub 2013 Mar, 7. PMID:23478062<ref>PMID:23478062</ref>
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[[http://www.uniprot.org/uniprot/DPO3A_THEAQ DPO3A_THEAQ]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4iqj]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQJ OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:023478062</ref><references group="xtra"/><references/>
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*[[DNA polymerase|DNA polymerase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: DNA-directed DNA polymerase]]
[[Category: DNA-directed DNA polymerase]]
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Lin, J.]]
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[[Category: Lin, J]]
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[[Category: Liu, B.]]
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[[Category: Liu, B]]
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[[Category: Steitz, T.]]
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[[Category: Steitz, T]]
[[Category: Alpha subunit]]
[[Category: Alpha subunit]]
[[Category: Dna replication]]
[[Category: Dna replication]]

Revision as of 12:13, 21 December 2014

Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome

4iqj, resolution 3.20Å

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