4ibn
From Proteopedia
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| - | + | ==Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif== | |
| - | + | <StructureSection load='4ibn' size='340' side='right' caption='[[4ibn]], [[Resolution|resolution]] 1.62Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4ibn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_organism Uncultured organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IBN FirstGlance]. <br> | ||
| + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ibn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ibn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ibn RCSB], [http://www.ebi.ac.uk/pdbsum/4ibn PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from alphaA-helix to beta1-strand immediately next to the first aspartate residue, as in the active site of RNase H2. However, the structure and enzymatic properties of LC9-RNase H1 highly resemble those of RNase H1, instead of RNase H2. We propose that LC9-RNase H1 represents bacterial RNases H1 with an atypical DEDN active site motif, which are evolutionarily distinct from those with a typical DEDD active site motif. | ||
| - | + | Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.,Nguyen TN, You DJ, Kanaya E, Koga Y, Kanaya S FEBS Lett. 2013 May 2;587(9):1418-23. doi: 10.1016/j.febslet.2013.03.020. Epub, 2013 Mar 20. PMID:23523920<ref>PMID:23523920</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Ribonuclease|Ribonuclease]] | ||
| + | *[[Temp|Temp]] | ||
| + | *[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Ribonuclease H]] | [[Category: Ribonuclease H]] | ||
[[Category: Uncultured organism]] | [[Category: Uncultured organism]] | ||
| - | [[Category: Kanaya, E | + | [[Category: Kanaya, E]] |
| - | [[Category: Kanaya, S | + | [[Category: Kanaya, S]] |
| - | [[Category: Koga, Y | + | [[Category: Koga, Y]] |
| - | [[Category: Nguyen, T N | + | [[Category: Nguyen, T N]] |
| - | [[Category: You, D J | + | [[Category: You, D J]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Hydrolases']] | [[Category: Hydrolases']] | ||
[[Category: Ribonuclease h]] | [[Category: Ribonuclease h]] | ||
[[Category: Rnase h]] | [[Category: Rnase h]] | ||
Revision as of 12:16, 21 December 2014
Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif
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Categories: Ribonuclease H | Uncultured organism | Kanaya, E | Kanaya, S | Koga, Y | Nguyen, T N | You, D J | Hydrolase | Hydrolases' | Ribonuclease h | Rnase h
