4hh0
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides== | |
| - | + | <StructureSection load='4hh0' size='340' side='right' caption='[[4hh0]], [[Resolution|resolution]] 2.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4hh0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HH0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HH0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hh1|4hh1]], [[4hh3|4hh3]], [[4hh2|4hh2]], [[2iyg|2iyg]], [[2iyi|2iyi]], [[1yrx|1yrx]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">appA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hh0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hh0 RCSB], [http://www.ebi.ac.uk/pdbsum/4hh0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources, depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the bacterium for using light energy. Illumination, however, enhances repression under semiaerobic conditions. Here, we describe molecular details of two proteins mediating oxygen and light control of photosynthesis-gene expression: the light-sensing antirepressor AppA and the transcriptional repressor PpsR. Our crystal structures of both proteins and their complex and hydrogen/deuterium-exchange data show that light activation of AppA-PpsR2 affects the PpsR effector region within the complex. DNA binding studies demonstrate the formation of a light-sensitive ternary AppA-PpsR-DNA complex. We discuss implications of these results for regulation by light and oxygen, highlighting new insights into blue light-mediated signal transduction. | ||
| - | + | A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.,Winkler A, Heintz U, Lindner R, Reinstein J, Shoeman RL, Schlichting I Nat Struct Mol Biol. 2013 Jul;20(7):859-67. doi: 10.1038/nsmb.2597. Epub 2013 Jun, 2. PMID:23728293<ref>PMID:23728293</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Rhodobacter sphaeroides]] | [[Category: Rhodobacter sphaeroides]] | ||
| - | [[Category: Heintz, U | + | [[Category: Heintz, U]] |
| - | [[Category: Lindner, R | + | [[Category: Lindner, R]] |
| - | [[Category: Reinstein, J | + | [[Category: Reinstein, J]] |
| - | [[Category: Schlichting, I | + | [[Category: Schlichting, I]] |
| - | [[Category: Shoeman, R | + | [[Category: Shoeman, R]] |
| - | [[Category: Winkler, A | + | [[Category: Winkler, A]] |
[[Category: Bluf domain]] | [[Category: Bluf domain]] | ||
[[Category: Flavoprotein]] | [[Category: Flavoprotein]] | ||
Revision as of 12:17, 21 December 2014
Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides
| |||||||||||
