1s32
From Proteopedia
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| - | [[Image:1s32.gif|left|200px]] | + | [[Image:1s32.gif|left|200px]] |
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| - | '''Molecular Recognition of the Nucleosomal 'Supergroove'''' | + | {{Structure |
| + | |PDB= 1s32 |SIZE=350|CAPTION= <scene name='initialview01'>1s32</scene>, resolution 2.05Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IMT:4-AMINO-(1-METHYLIMIDAZOLE)-2-CARBOXYLIC+ACID'>IMT</scene> and <scene name='pdbligand=OGG:2-(2-CARBAMOYLMETHOXY-ETHOXY)-ACETAMIDE'>OGG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= Histone H3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), Histone H4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), Histone H2A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), Histone H2B.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]) | ||
| + | }} | ||
| + | |||
| + | '''Molecular Recognition of the Nucleosomal 'Supergroove'''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S32 is a [ | + | 1S32 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S32 OCA]. |
==Reference== | ==Reference== | ||
| - | Molecular recognition of the nucleosomal "supergroove"., Edayathumangalam RS, Weyermann P, Gottesfeld JM, Dervan PB, Luger K, Proc Natl Acad Sci U S A. 2004 May 4;101(18):6864-9. Epub 2004 Apr 20. PMID:[http:// | + | Molecular recognition of the nucleosomal "supergroove"., Edayathumangalam RS, Weyermann P, Gottesfeld JM, Dervan PB, Luger K, Proc Natl Acad Sci U S A. 2004 May 4;101(18):6864-9. Epub 2004 Apr 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15100411 15100411] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
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[[Category: clamp]] | [[Category: clamp]] | ||
[[Category: nucleosome core particle (ncp)]] | [[Category: nucleosome core particle (ncp)]] | ||
| - | [[Category: nucleosome | + | [[Category: nucleosome dynamic]] |
[[Category: pyrrole-imidazole (py-im) hairpin polyamide]] | [[Category: pyrrole-imidazole (py-im) hairpin polyamide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:59:48 2008'' |
Revision as of 11:59, 20 March 2008
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| , resolution 2.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | Histone H3 (Xenopus laevis), Histone H4 (Xenopus laevis), Histone H2A (Xenopus laevis), Histone H2B.1 (Xenopus laevis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Molecular Recognition of the Nucleosomal 'Supergroove'
Overview
Chromatin is the physiological substrate in all processes involving eukaryotic DNA. By organizing 147 base pairs of DNA into two tight superhelical coils, the nucleosome generates an architecture where DNA regions that are 80 base pairs apart on linear DNA are brought into close proximity, resulting in the formation of DNA "supergrooves." Here, we report the design of a hairpin polyamide dimer that targets one such supergroove. The 2-A crystal structure of the nucleosome-polyamide complex shows that the bivalent "clamp" effectively crosslinks the two gyres of the DNA superhelix, improves positioning of the DNA on the histone octamer, and stabilizes the nucleosome against dissociation. Our findings identify nucleosomal supergrooves as platforms for molecular recognition of condensed eukaryotic DNA. In vivo, supergrooves may foster synergistic protein-protein interactions by bringing two regulatory elements into juxtaposition. Because supergroove formation is independent of the translational position of the DNA on the histone octamer, accurate nucleosome positioning over regulatory elements is not required for supergroove participation in eukaryotic gene regulation.
About this Structure
1S32 is a Protein complex structure of sequences from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Molecular recognition of the nucleosomal "supergroove"., Edayathumangalam RS, Weyermann P, Gottesfeld JM, Dervan PB, Luger K, Proc Natl Acad Sci U S A. 2004 May 4;101(18):6864-9. Epub 2004 Apr 20. PMID:15100411
Page seeded by OCA on Thu Mar 20 13:59:48 2008
