1s47
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1s47.gif|left|200px]] | + | [[Image:1s47.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure analysis of the DNA quadruplex d(TGGGGT)S2''' | + | {{Structure |
| + | |PDB= 1s47 |SIZE=350|CAPTION= <scene name='initialview01'>1s47</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=TL:THALLIUM (I) ION'>TL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure analysis of the DNA quadruplex d(TGGGGT)S2''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1S47 is a [ | + | 1S47 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S47 OCA]. |
==Reference== | ==Reference== | ||
| - | A thymine tetrad in d(TGGGGT) quadruplexes stabilized with Tl+/Na+ ions., Caceres C, Wright G, Gouyette C, Parkinson G, Subirana JA, Nucleic Acids Res. 2004 Feb 11;32(3):1097-102. Print 2004. PMID:[http:// | + | A thymine tetrad in d(TGGGGT) quadruplexes stabilized with Tl+/Na+ ions., Caceres C, Wright G, Gouyette C, Parkinson G, Subirana JA, Nucleic Acids Res. 2004 Feb 11;32(3):1097-102. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14960719 14960719] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Caceres, C.]] | [[Category: Caceres, C.]] | ||
| Line 21: | Line 30: | ||
[[Category: quadruplex dna]] | [[Category: quadruplex dna]] | ||
[[Category: thallium]] | [[Category: thallium]] | ||
| - | [[Category: thymine triads and | + | [[Category: thymine triads and tetrad]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:00:12 2008'' |
Revision as of 12:00, 20 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure analysis of the DNA quadruplex d(TGGGGT)S2
Overview
We report two new structures of the quadruplex d(TGGGGT)4 obtained by single crystal X-ray diffraction. In one of them a thymine tetrad is found. Thus the yeast telomere sequences d(TG1-3) might be able to form continuous quadruplex structures, involving both guanine and thymine tetrads. Our study also shows substantial differences in the arrangement of thymines when compared with previous studies. We find five different types of organization: (i) groove binding with hydrogen bonds to guanines from a neighbour quadruplex; (ii) partially ordered groove binding, without any hydrogen bond; (iii) stacked thymine triads, formed at the 3'ends of the quadruplexes; (iv) a thymine tetrad between two guanine tetrads. Thymines are stabilized in pairs by single hydrogen bonds. A central sodium ion interacts with two thymines and contributes to the tetrad structure. (v) Completely disordered thymines which do not show any clear location in the crystal. The tetrads are stabilized by either Na+ or Tl+ ions. We show that by using MAD methods, Tl+ can be unambiguously located and distinguished from Na+. We can thus determine the preference for either ion in each ionic site of the structure under the conditions used by us.
About this Structure
1S47 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A thymine tetrad in d(TGGGGT) quadruplexes stabilized with Tl+/Na+ ions., Caceres C, Wright G, Gouyette C, Parkinson G, Subirana JA, Nucleic Acids Res. 2004 Feb 11;32(3):1097-102. Print 2004. PMID:14960719
Page seeded by OCA on Thu Mar 20 14:00:12 2008
