4hf2

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{{STRUCTURE_4hf2| PDB=4hf2 | SCENE= }}
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==Crystal Structure of E43A IscR mutant bound to its promoter==
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===Crystal Structure of E43A IscR mutant bound to its promoter===
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<StructureSection load='4hf2' size='340' side='right' caption='[[4hf2]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23644595}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4hf2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HF2 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hf0|4hf0]], [[4hf1|4hf1]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">iscR, yfhP, b2531, JW2515 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4hf2 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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IscR from Escherichia coli is an unusual metalloregulator in that both apo and iron sulfur (Fe-S)-IscR regulate transcription and exhibit different DNA binding specificities. Here, we report structural and biochemical studies of IscR suggesting that remodeling of the protein-DNA interface upon Fe-S ligation broadens the DNA binding specificity of IscR from binding the type 2 motif only to both type 1 and type 2 motifs. Analysis of an apo-IscR variant with relaxed target-site discrimination identified a key residue in wild-type apo-IscR that, we propose, makes unfavorable interactions with a type 1 motif. Upon Fe-S binding, these interactions are apparently removed, thereby allowing holo-IscR to bind both type 1 and type 2 motifs. These data suggest a unique mechanism of ligand-mediated DNA site recognition, whereby metallocluster ligation relocates a protein-specificity determinant to expand DNA target-site selection, allowing a broader transcriptomic response by holo-IscR.
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==Function==
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Studies of IscR reveal a unique mechanism for metal-dependent regulation of DNA binding specificity.,Rajagopalan S, Teter SJ, Zwart PH, Brennan RG, Phillips KJ, Kiley PJ Nat Struct Mol Biol. 2013 Jun;20(6):740-7. doi: 10.1038/nsmb.2568. Epub 2013 May , 5. PMID:23644595<ref>PMID:23644595</ref>
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[[http://www.uniprot.org/uniprot/ISCR_ECOLI ISCR_ECOLI]] Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA.<ref>PMID:11742080</ref> <ref>PMID:16824106</ref> <ref>PMID:20421606</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4hf2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HF2 OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023644595</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
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[[Category: Phillips, K J.]]
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[[Category: Phillips, K J]]
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[[Category: Rajagopalan, S R.]]
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[[Category: Rajagopalan, S R]]
[[Category: Dna binding]]
[[Category: Dna binding]]
[[Category: Iron-sulfur cluster]]
[[Category: Iron-sulfur cluster]]

Revision as of 12:36, 21 December 2014

Crystal Structure of E43A IscR mutant bound to its promoter

4hf2, resolution 2.99Å

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