1s5h
From Proteopedia
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| - | [[Image:1s5h.gif|left|200px]] | + | [[Image:1s5h.gif|left|200px]] |
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| - | '''Potassium Channel Kcsa-Fab Complex T75C mutant in K+''' | + | {{Structure |
| + | |PDB= 1s5h |SIZE=350|CAPTION= <scene name='initialview01'>1s5h</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene> and <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= KCSA, SKC1, SCO7660, SC10F4.33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Streptomyces coelicolor, and Streptomyces lividans]) | ||
| + | }} | ||
| + | |||
| + | '''Potassium Channel Kcsa-Fab Complex T75C mutant in K+''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S5H is a [ | + | 1S5H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_coelicolor,_and_streptomyces_lividans Streptomyces coelicolor, and streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5H OCA]. |
==Reference== | ==Reference== | ||
| - | A mutant KcsA K(+) channel with altered conduction properties and selectivity filter ion distribution., Zhou M, MacKinnon R, J Mol Biol. 2004 May 7;338(4):839-46. PMID:[http:// | + | A mutant KcsA K(+) channel with altered conduction properties and selectivity filter ion distribution., Zhou M, MacKinnon R, J Mol Biol. 2004 May 7;338(4):839-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15099749 15099749] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: selectivity filter]] | [[Category: selectivity filter]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:00:43 2008'' |
Revision as of 12:00, 20 March 2008
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| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | KCSA, SKC1, SCO7660, SC10F4.33 (Streptomyces coelicolor, and Streptomyces lividans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Potassium Channel Kcsa-Fab Complex T75C mutant in K+
Overview
The selectivity filter of K(+) channels is comprised of a linear queue of four equal-spaced ion-binding sites spanning a distance of 12A. Each site is formed of eight oxygen atoms from the protein. The first three sites, numbered 1-3 from the extracellular side, are made of exclusively main-chain carbonyl oxygen atoms. The fourth site, closest to the intracellular side, is made of four main-chain carbonyl oxygen atoms and four threonine side-chain hydroxyl oxygen atoms. Here we characterize the effects of mutating the threonine to cysteine on the distribution of ions in the selectivity filter and on the conduction of ions through the filter. The mutation influences the occupancy of K(+) at sites 2 and 4 and it reduces the maximum rate of conduction in the limit of high K(+) concentration. The mutation does not affect the conduction of Rb(+). These results can be understood in the context of a conduction mechanism in which a pair of K ions switch between energetically balanced 1,3 and 2,4 configurations.
About this Structure
1S5H is a Single protein structure of sequence from Mus musculus and Streptomyces coelicolor, and streptomyces lividans. Full crystallographic information is available from OCA.
Reference
A mutant KcsA K(+) channel with altered conduction properties and selectivity filter ion distribution., Zhou M, MacKinnon R, J Mol Biol. 2004 May 7;338(4):839-46. PMID:15099749
Page seeded by OCA on Thu Mar 20 14:00:43 2008
