4iul
From Proteopedia
(Difference between revisions)
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| - | + | ==MIF4G domain of DAP5== | |
| - | === | + | <StructureSection load='4iul' size='340' side='right' caption='[[4iul]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4iul]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IUL FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DAP5, EIF4G2, OK/SW-cl.75 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iul OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iul RCSB], [http://www.ebi.ac.uk/pdbsum/4iul PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Death-associated protein 5 (DAP5/p97) is a homolog of the eukaryotic initiation factor 4G (eIF4G) that promotes the IRES-driven translation of multiple cellular mRNAs. Central to its function is the middle domain (MIF4G), which recruits the RNA helicase eIF4A. The middle domain of eIF4G consists of tandem HEAT repeats that coalesce to form a solenoid-type structure. Here, we report the crystal structure of the DAP5 MIF4G domain. Its overall fold is very similar to that of eIF4G; however, significant conformational variations impart distinct surface properties that could explain the observed differences in IRES binding between the two proteins. Interestingly, quantitative analysis of the DAP5-eIF4A interaction using isothermal titration calorimetry reveals a 10-fold lower affinity than with the eIF4G-eIF4A interaction that appears to affect their ability to stimulate eIF4A RNA unwinding activity in vitro. This difference in stability of the complex may have functional implications in selecting the mode of translation initiation. | ||
| - | + | Structural Analysis of the DAP5 MIF4G Domain and Its Interaction with eIF4A.,Virgili G, Frank F, Feoktistova K, Sawicki M, Sonenberg N, Fraser CS, Nagar B Structure. 2013 Mar 5. pii: S0969-2126(13)00023-3. doi:, 10.1016/j.str.2013.01.015. PMID:23478064<ref>PMID:23478064</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | <references | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Feoktistova, K | + | [[Category: Feoktistova, K]] |
| - | [[Category: Frank, F | + | [[Category: Frank, F]] |
| - | [[Category: Fraser, C | + | [[Category: Fraser, C]] |
| - | [[Category: Nagar, B | + | [[Category: Nagar, B]] |
| - | [[Category: Sawicki, M | + | [[Category: Sawicki, M]] |
| - | [[Category: Sonenberg, N | + | [[Category: Sonenberg, N]] |
| - | [[Category: Virgili, G | + | [[Category: Virgili, G]] |
[[Category: Eif4a]] | [[Category: Eif4a]] | ||
[[Category: Heat repeat]] | [[Category: Heat repeat]] | ||
[[Category: Protein-protein interaction]] | [[Category: Protein-protein interaction]] | ||
[[Category: Translation]] | [[Category: Translation]] | ||
Revision as of 12:44, 21 December 2014
MIF4G domain of DAP5
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