1s6l
From Proteopedia
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| - | [[Image:1s6l.gif|left|200px]] | + | [[Image:1s6l.gif|left|200px]] |
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| - | '''Solution structure of MerB, the Organomercurial Lyase involved in the bacterial mercury resistance system''' | + | {{Structure |
| + | |PDB= 1s6l |SIZE=350|CAPTION= <scene name='initialview01'>1s6l</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Alkylmercury_lyase Alkylmercury lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.99.1.2 4.99.1.2] | ||
| + | |GENE= MERB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of MerB, the Organomercurial Lyase involved in the bacterial mercury resistance system''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S6L is a [ | + | 1S6L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6L OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structural studies reveal a novel protein fold for MerB, the organomercurial lyase involved in the bacterial mercury resistance system., Di Lello P, Benison GC, Valafar H, Pitts KE, Summers AO, Legault P, Omichinski JG, Biochemistry. 2004 Jul 6;43(26):8322-32. PMID:[http:// | + | NMR structural studies reveal a novel protein fold for MerB, the organomercurial lyase involved in the bacterial mercury resistance system., Di Lello P, Benison GC, Valafar H, Pitts KE, Summers AO, Legault P, Omichinski JG, Biochemistry. 2004 Jul 6;43(26):8322-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15222745 15222745] |
[[Category: Alkylmercury lyase]] | [[Category: Alkylmercury lyase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:12 2008'' |
Revision as of 12:01, 20 March 2008
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| Gene: | MERB (Escherichia coli) | ||||||
| Activity: | Alkylmercury lyase, with EC number 4.99.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of MerB, the Organomercurial Lyase involved in the bacterial mercury resistance system
Overview
Mercury resistant bacteria have developed a system of two enzymes (MerA and MerB), which allows them to efficiently detoxify both ionic and organomercurial compounds. The organomercurial lyase (MerB) catalyzes the protonolysis of the carbon-mercury bond resulting in the formation of ionic mercury and a reduced hydrocarbon. The ionic mercury [Hg(II)] is subsequently reduced to the less reactive elemental mercury [Hg(0)] by a specific mercuric reductase (MerA). To better understand MerB's unique enzymatic activity, we used nuclear magnetic resonance (NMR) spectroscopy to determine the structure of the free enzyme. MerB is characterized by a novel protein fold consisting of three noninteracting antiparallel beta-sheets surrounded by six alpha-helices. By comparing the NMR data of free MerB and the MerB/Hg/DTT complex, we identified a set of residues that likely define a Hg/DTT binding site. These residues cluster around two cysteines (C(96) and C(159)) that are crucial to MerB's catalytic activity. A detailed analysis of the structure revealed the presence of an extensive hydrophobic groove adjacent to this Hg/DTT binding site. This extensive hydrophobic groove has the potential to interact with the hydrocarbon moiety of a wide variety of substrates and may explain the broad substrate specificity of MerB.
About this Structure
1S6L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
NMR structural studies reveal a novel protein fold for MerB, the organomercurial lyase involved in the bacterial mercury resistance system., Di Lello P, Benison GC, Valafar H, Pitts KE, Summers AO, Legault P, Omichinski JG, Biochemistry. 2004 Jul 6;43(26):8322-32. PMID:15222745
Page seeded by OCA on Thu Mar 20 14:01:12 2008
