1s7g

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[[Image:1s7g.gif|left|200px]]<br /><applet load="1s7g" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1s7g.gif|left|200px]]
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caption="1s7g, resolution 2.30&Aring;" />
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'''Structural Basis for the Mechanism and Regulation of Sir2 Enzymes'''<br />
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{{Structure
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|PDB= 1s7g |SIZE=350|CAPTION= <scene name='initialview01'>1s7g</scene>, resolution 2.30&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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|ACTIVITY=
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|GENE= NPDA2, AF0112 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])
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}}
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'''Structural Basis for the Mechanism and Regulation of Sir2 Enzymes'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1S7G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NAD:'>NAD</scene>, <scene name='pdbligand=APR:'>APR</scene>, <scene name='pdbligand=2PE:'>2PE</scene>, <scene name='pdbligand=P6G:'>P6G</scene>, <scene name='pdbligand=PG4:'>PG4</scene>, <scene name='pdbligand=1PE:'>1PE</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7G OCA].
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1S7G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7G OCA].
==Reference==
==Reference==
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Structural basis for the mechanism and regulation of Sir2 enzymes., Avalos JL, Boeke JD, Wolberger C, Mol Cell. 2004 Mar 12;13(5):639-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15023335 15023335]
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Structural basis for the mechanism and regulation of Sir2 enzymes., Avalos JL, Boeke JD, Wolberger C, Mol Cell. 2004 Mar 12;13(5):639-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15023335 15023335]
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: sirtuin]]
[[Category: sirtuin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:58:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:29 2008''

Revision as of 12:01, 20 March 2008

Image:1s7g.gif


PDB ID 1s7g

Drag the structure with the mouse to rotate
, resolution 2.30Å
Ligands: , , , , , , , and
Gene: NPDA2, AF0112 (Archaeoglobus fulgidus)
Coordinates: save as pdb, mmCIF, xml



Structural Basis for the Mechanism and Regulation of Sir2 Enzymes


Overview

Sir2 proteins form a family of NAD(+)-dependent protein deacetylases required for diverse biological processes, including transcriptional silencing, suppression of rDNA recombination, control of p53 activity, regulation of acetyl-CoA synthetase, and aging. Although structures of Sir2 enzymes in the presence and absence of peptide substrate or NAD(+) have been determined, the role of the enzyme in the mechanism of deacetylation and NAD(+) cleavage is still unclear. Here, we present additional structures of Sir2Af2 in several differently complexed states: in a productive complex with NAD(+), in a nonproductive NAD(+) complex with bound ADP-ribose, and in the unliganded state. We observe a new mode of NAD(+) binding that seems to depend on acetyl-lysine binding, in which the nicotinamide ring of NAD(+) is buried in the highly conserved "C" pocket of the enzyme. We propose a detailed structure-based mechanism for deacetylation and nicotinamide inhibition of Sir2 consistent with mutagenesis and enzymatic studies.

About this Structure

1S7G is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structural basis for the mechanism and regulation of Sir2 enzymes., Avalos JL, Boeke JD, Wolberger C, Mol Cell. 2004 Mar 12;13(5):639-48. PMID:15023335

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