4isr
From Proteopedia
(Difference between revisions)
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| - | + | ==Binding domain of Botulinum neurotoxin DC in complex with rat synaptotagmin II== | |
| - | + | <StructureSection load='4isr' size='340' side='right' caption='[[4isr]], [[Resolution|resolution]] 2.59Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4isr]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ISR FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4isq|4isq]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4isr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4isr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4isr RCSB], [http://www.ebi.ac.uk/pdbsum/4isr PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Botulinum neurotoxins (BoNTs) can cause paralysis at exceptionally low concentrations and include seven serotypes (BoNT/A-G). The chimeric BoNT/DC toxin has a receptor binding domain similar to the same region in BoNT/C. However, BoNT/DC does not share protein receptor with BoNT/C. Instead, it shares synaptotagmin (Syt) I and II as receptors with BoNT/B, despite their low sequence similarity. Here, we present the crystal structures of the binding domain of BoNT/DC in complex with the recognition domains of its protein receptors, Syt-I and Syt-II. The structures reveal that BoNT/DC possesses a Syt binding site, distinct from the established Syt-II binding site in BoNT/B. Structure-based mutagenesis further shows that hydrophobic interactions play a key role in Syt binding. The structures suggest that the BoNT/DC ganglioside binding sites are independent of the protein receptor binding site. Our results reveal the remarkable versatility in the receptor recognition of the BoNTs. | ||
| - | + | Crystal Structures of Botulinum Neurotoxin DC in Complex with Its Protein Receptors Synaptotagmin I and II.,Berntsson RP, Peng L, Svensson LM, Dong M, Stenmark P Structure. 2013 Sep 3;21(9):1602-11. doi: 10.1016/j.str.2013.06.026. Epub 2013, Aug 8. PMID:23932591<ref>PMID:23932591</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Clostridium botulinum]] | [[Category: Clostridium botulinum]] | ||
| - | [[Category: Berntsson, R P.A | + | [[Category: Berntsson, R P.A]] |
| - | [[Category: Dong, M | + | [[Category: Dong, M]] |
| - | [[Category: Peng, L | + | [[Category: Peng, L]] |
| - | [[Category: Stenmark, P | + | [[Category: Stenmark, P]] |
| - | [[Category: Svensson, L M | + | [[Category: Svensson, L M]] |
[[Category: Membrane binding]] | [[Category: Membrane binding]] | ||
[[Category: Synaptotagmin and ganglioside binding]] | [[Category: Synaptotagmin and ganglioside binding]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Revision as of 12:57, 21 December 2014
Binding domain of Botulinum neurotoxin DC in complex with rat synaptotagmin II
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