This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1s7z
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1s7z.jpg|left|200px]] | + | [[Image:1s7z.jpg|left|200px]] |
| - | + | ||
| - | '''Structure of Ocr from Bacteriophage T7''' | + | {{Structure |
| + | |PDB= 1s7z |SIZE=350|CAPTION= <scene name='initialview01'>1s7z</scene>, resolution 1.83Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CS:CESIUM ION'>CS</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= 0.3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T7]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Ocr from Bacteriophage T7''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1S7Z is a [ | + | 1S7Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http:// | + | Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11804597 11804597] |
[[Category: Bacteriophage t7]] | [[Category: Bacteriophage t7]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 34: | ||
[[Category: gene regulation]] | [[Category: gene regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:39 2008'' |
Revision as of 12:01, 20 March 2008
| |||||||
| , resolution 1.83Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | 0.3 (Bacteriophage T7) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Ocr from Bacteriophage T7
Overview
We have solved, by X-ray crystallography to a resolution of 1.8 A, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA.
About this Structure
1S7Z is a Single protein structure of sequence from Bacteriophage t7. Full crystallographic information is available from OCA.
Reference
Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597
Page seeded by OCA on Thu Mar 20 14:01:39 2008
