1s7z
From Proteopedia
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| - | [[Image:1s7z.jpg|left|200px]] | + | [[Image:1s7z.jpg|left|200px]] |
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| - | '''Structure of Ocr from Bacteriophage T7''' | + | {{Structure |
| + | |PDB= 1s7z |SIZE=350|CAPTION= <scene name='initialview01'>1s7z</scene>, resolution 1.83Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CS:CESIUM ION'>CS</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= 0.3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T7]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Ocr from Bacteriophage T7''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S7Z is a [ | + | 1S7Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http:// | + | Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11804597 11804597] |
[[Category: Bacteriophage t7]] | [[Category: Bacteriophage t7]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: gene regulation]] | [[Category: gene regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:39 2008'' |
Revision as of 12:01, 20 March 2008
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| , resolution 1.83Å | |||||||
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| Ligands: | |||||||
| Gene: | 0.3 (Bacteriophage T7) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Ocr from Bacteriophage T7
Overview
We have solved, by X-ray crystallography to a resolution of 1.8 A, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA.
About this Structure
1S7Z is a Single protein structure of sequence from Bacteriophage t7. Full crystallographic information is available from OCA.
Reference
Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597
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